miR-106b在鼻咽癌组织中表达及对鼻咽癌CNE-2细胞生物学特性的影响  

Expression of miR-106b in nasopharyngeal carcinoma tissues and its effects on the biological characteristics of nasopharyngeal carcinoma CNE-2 cells

作　　者：周士霞[1] 王海莉[1] 王豪勋[1] 

机构地区：[1]郑州大学第二附属医院,郑州450014

出　　处：《山东医药》2017年第20期9-12,共4页Shandong Medical Journal

基　　金：河南省科技发展计划(142102310087)

摘　　要：目的探讨miR-106b在鼻咽癌组织中的表达及其对鼻咽癌CNE-2细胞生物学特性的影响。方法选取68例份初治鼻咽癌患者的手术切除标本和45例份鼻咽炎患者的鼻咽部活检组织标本,采用实时荧光定量PCR法检测miR-106b表达,分析miR-106b表达与鼻咽癌患者临床病理参数的关系。取体外培养的对数生长期人鼻咽癌细胞株CNE-2分为四组(1×106个/组)。miR-106b模拟物组、miR-106b抑制物组分别转染miR-106b模拟物及miR-106b抑制物序列,阴性对照组转染阴性对照序列,空白对照组不处理;采用实时荧光定量PCR技术检测各组miR-106b表达,MTT法检测各组细胞增殖情况(以吸光度值表示),流式细胞仪检测各组细胞周期,Transwell试验检测各组细胞迁移和侵袭能力。结果鼻咽癌及鼻咽炎组织miR-106b相对表达量分别为1.57±0.15、1.14±0.12,二者比较P<0.05;miR-106b相对表达量与鼻咽癌患者淋巴结转移、侵犯颈动脉鞘和侵犯颅底有关(P均<0.05)。各组miR-106b相对表达量:miR-106b模拟物组高于miR-106b抑制物组、阴性对照组和空白对照组,miR-106b抑制物组低于阴性对照组和空白对照组,P均<0.05;各组细胞增殖情况:miR-106b模拟物组细胞接种后24、48、72和96 h时吸光度值均高于miR-106b抑制物组、阴性对照组和空白对照组,而miR-106b抑制物组低于阴性对照组和空白对照组,P均<0.05;各组细胞周期:miR-106b模拟物组S期细胞比例高于miR-106b抑制物组、阴性对照组和空白对照组,而miR-106b抑制物组细胞S期比例低于阴性对照组和空白对照组,P均<0.05;各组细胞迁移和侵袭情况:miR-106b模拟物组迁移和侵袭细胞数均高于miR-106b抑制物组、阴性对照组和空白对照组,且miR-106b抑制物组均低于阴性对照组和空白对照组,P均<0.05。结论 miR-106b在鼻咽癌组织中呈高表达;miR-106b过表达可促进鼻咽癌CNE-2细胞增殖、迁移和侵袭能力。Objective To investigate the expression of miR-106b in nasopharyngeal＇ carcinoma tissues and its effects on the biological characteristics of nasopharyngeal carcinoma CNE-2 cells. Methods Sixty-eight cases of surgical resection specimens from first treatment patients with nasopharyngeal carcinoma and 45 cases of nas0pharyngeal biopsy specimens from patients with chronic nasopharyngitis were selected. The expression of miR-106b was detected by using real-time PCR technology. The relationship between the expression of miR-106b and elinicopathological parameters in patients with nasopharyngeal carcinoma was analyzed. The nasopharyngeal carcinoma CNE-2 cells in the logarithmic growth phase were divided into four groups （ 1 × 10^6 cells/group）. Cells in the miR-106b mimics group and miR-106b inhibitor group were transected with miR-106b mimics and miR-106 inhibitor, respectively. Ceils in the negative control group were transfected with negative control sequence, while cells in the blank control group were not treated. The cell proliferation of each transfected group was tested by using MTr assay. The cell cycle of each transfected group was detected by using flow cytometry. The cell migration and invasion of each transfected group were detected by using Transwell experiment. Results The relative expression levels of miR-106b in the nasopharyngeal carcinoma tissues and chronic nasopharyngitis nasopharyngeal tissues were 1.57 ± 0.15 and 1.14 ±0.12, respectively, P 〈 0.05. The expression of miR-106b in the nasopharyngeal carcinoma tissues was related with lymph node metastasis, carotid sheath violations, and skull base violations （ all P 〈 0.05 ）. The relative expression level in each transfection group： the miR-106b mimic group was significantly higher than the miR-106b inhibitor group, the negative control group and blank control group, and the miR-106b inhibitor group was lower than the negative control group and blank control group, all P 〈 0.05. The proliferation of each transfection group ：

关 键 词：鼻咽癌 微小RNA-106b CNE-2细胞 细胞增殖 细胞迁移 

分 类 号：R739.6[医药卫生—肿瘤]