多氯联苯在两种海洋微藻中毒性效应及富集效应的研究  

A study of toxicity and bioaccumulation of PCBs in two species of marine microalgae

作　　者：吴越[1] 陈星星[1] 潘齐存 郑伊诺 胡园[1] 王瑶华[1] 陆荣茂[1] 黄振华[1] 

机构地区：[1]浙江省海洋水产养殖研究所浙江省近岸水域开发与保护重点实验室,浙江温州325005

出　　处：《海洋科学》2017年第3期61-67,共7页Marine Sciences

基　　金：浙江省近岸水域生物资源开发与保护重点实验室开放基金项目(J2013010);浙江省公益技术研究农业项目(2014C32079);浙江省科技计划项目(2015F30021)~~

摘　　要：通过分析2,2',3,4,4',5'-六氯联苯(PCB_(138))对牟氏角毛藻(Chaetoceros muelleri)和亚心形扁藻(Platymonas subcordiformis)生长的影响,以及PCB_(138)对两种微藻超氧化物歧化酶(Superoxide dismutase,SOD)活性的影响,研究多氯联苯对海洋微藻的毒性效应,并采用气相色谱法研究了两种微藻对PCB_(138)的生物富集效应。结果表明,PCB_(138)对牟氏角毛藻和亚心形扁藻均有明显的毒性效应作用,对2种藻类的96 h半抑制质量浓度(96hEC_(50))分别为0.057 mg/和0.354 mg/L,与亚心形扁藻相比,牟氏角毛藻对PCB_(138)更敏感,耐受性更差;低浓度的PCB_(138)对两种微藻SOD酶活性有诱导作用,但当PCB_(138)浓度过高时,SOD酶活性下降;两种微藻对PCB_(138)均有明显的富集作用,随着培养时间的延长,牟氏角毛藻中富集的PCB_(138)含量呈上升趋势,而亚心形扁藻中PCB_(138)含量呈现先上升后下降趋势。In order to investigate the toxicity effects of PCBs on marine microalgae, two algae species, Chaetoceros muelleri and Platymonas subcordiformis, were selected to study the effects of PCB138 on growth and SOD enzyme activities. Meanwhile, the enrichment effects of PCB138 on C. muelleri and P. subcordiformis were analyzed by gas chromatography. The results showed that PCB138 had obvious toxicity effects on the growth of C. muelleri and P. subcordiformis. The 96-h EC50 of PCB138 on C. muelleri and P. subcordiformis were 0.057 mg·L-1 and 0.354 mg·L-1, respectively, which indicated that C. muelleri showed more sensitivity and poorer tolerance to PCB138 than P. subcordiformis. SOD activities in two microalgae cells were greatly induced by low levels of PCB138, and reduced as the PCB138 concentrations increased above threshold values. Both of the algae species had strong capabilities to accumulate PCB138. The adsorption amount of PCB138 in C. muelleri increased with increasing cultivation time, while the amount of PCB138 adsorbed in P. subcordiformis first increased, then decreased.

关 键 词：多氯联苯 海洋微藻 毒性效应 生物富集 

分 类 号：X171.5[环境科学与工程—环境科学]