炎症因子IL-23对黑色素瘤细胞增殖侵袭及双向分化的影响  

Effects of IL-23 on the herin proliferation,invasion and bi-differentiation of melanoma cells

作　　者：文明明[1] 马跃美[1] 赵秀兰[2,3] 张丹芳[2] 赵楠[2] 李岩磊[2] 林贤[2] 董学易[2] 刘芳[2] WEN Ming-ming MA Yue-mei ZHAO Xiu-lan ZHANG Dan-fang ZHAO Nan LI Yan-lei LIN Xian DONG Xue-yi LIU Fang(Department of Operative Surgery, Tianjin Medical University, Tianjin 300070, China Department of Pathology, Tianjin Medical University, Tianjin 300070, China Department of Pathology, General Hospital , Tianjin Medical University, Tianjin 300052, China)

机构地区：[1]天津医科大学外科手术学教研室,天津300070 [2]天津医科大学病理学教研室,天津300070 [3]天津医科大学总医院病理科,天津300052

出　　处：《天津医科大学学报》2017年第4期295-299,共5页Journal of Tianjin Medical University

基　　金：国家自然科学基金面上项目基金资助(81572872)

摘　　要：目的:检测白细胞介素-23(IL-23)在黑色素瘤中的表达并探讨IL-23对黑色素瘤细胞双向分化及增殖、侵袭能力的影响。方法:将IL-23降表达质粒转染至黑色素瘤细胞系MUM-2B中,使MUM-2B中IL-23外源性降表达,观察IL-23转染前、后MUM-2B细胞形态学改变;MTT、细胞划痕和Transwell侵袭实验检测对增殖、迁移及侵袭能力的影响;Western blot检测转染前、后MUM-2B中IL-23和相关蛋白(Vimentin、Snail、N-cadherin和MMP-2)表达变化情况;免疫荧光检测IL-23、Vimentin和MMP-2表达部位,明胶酶谱检测MMP-2、MMP-9活性。结果:转染后MUM-2B的细胞形态发生明显改变。细胞的增殖、迁移及侵袭能力显著减弱。IL-23降表达,N-cadherin、Vimentin、NF-κβ、Snail和MMP-2表达明显下调,MMP-2、MMP-9活性显著降低。结论:IL-23可促进黑色素瘤细胞向间叶样表型转变,并促进黑色素瘤细胞的增殖、迁移和侵袭。Objective：To examine the expression of IL-23 in melanoma cells and its effecton proliferation,invasion and bi-differentiation. Methods： To establish stable IL-23 knockdown cells, MUM-2B cells were stably transfected with sh-IL-23 and sh-Control vectors.The changes of MUM-2B cells morphology were detected after IL-23transfection by using microscope;The proliferation of MUM-2Bcells was determined by MTT,the migration ofMUM-2B cells was determined by the wound healing assay. And the invasion of MUM-2B cells was detected by Transwellexperiment.The changes of the expression of related protein（Vimentin,Snail,N-cadherin and MMP-2）in MUM-2Bcells were detected by Western blot after transfection ofIL-23;Using immunofluorescence, the sites of the expression of related protein（IL-23,Vimentin and MMP-2） were detected;The expression activities of MMP-2 and MMP-9 were analyzed by Gelatin Zymography. Results：After IL-23 knockdown, the morphology ofMUM-2B cells showed significant changes; IL-23 knockdown significantly decreased the abilities of MUM-2B cells to proliferate ,invade and migrate;Compared with MUM-2B-Control cells, knockdown expression of IL-23 significantly down-regulated N-cad, Vimentin, NF-κβ,Snailand MMP-2. Importantly, MUM-2B cells knockdown IL-23 demonstrated decreasedtheexpressionactivities of MMP-2 and MMP-9.Conclusion： IL-23 contributes to transfection of mesenchymal-like phenotypeand facilitates the proliferation,migration and invasionof melanoma cells.

关 键 词：黑色素瘤 IL-23 增殖 迁移 侵袭 

分 类 号：R36[医药卫生—病理学]