柴胡皂苷a影响大鼠急性脊髓损伤后机体炎症水平表达的实验研究  

Effect of saikosaponin a on expression of inflammation in rats after acute spinal cord injury

作　　者：朱双龙[1,2,3] 陈旭义[3] 段会全[1,2] 刘迎节[1,2] 孙中磊 徐云强[1,2] ZHU Shuang-long CHEN Xu-yi DUAN Hui-quan LIU Ying-jie SUN Zhong-lei XU Yun-qiang(Gradute School, Tianjin Medical University, Tianjin 300070,China Department of Orthopedic, General Hospital, Tianjin Medical University,Tianjin 300052,China Department of Brain, Affiliated Hospital of Logistics College of CAPF, Tianjin 300162, China ？？？Graduate School, Jinzhou Medical University, Jinzhou 121000, China)

机构地区：[1]天津医科大学研究生院,天津300070 [2]天津医科大学总医院骨科,天津300052 [3]武警后勤学院附属医院脑科中心,天津300162 [4]锦州医科大学研究生院,锦州121000

出　　处：《天津医科大学学报》2017年第4期300-303,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金青年基金资助项目(81101362,11102235).

摘　　要：目的:探讨不同浓度柴胡皂苷a对大鼠急性脊髓损伤早期组织和血清中肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)表达水平的影响。方法:选用健康成年SD大鼠30只,随机分为假手术组、单纯损伤组、柴胡皂苷a 5 mg/kg组、10 mg/kg组、20 mg/kg组共5组。利用改良Allen’s重物打击法制作急性脊髓损伤模型。单纯损伤组注入生理盐水,药物组通过腹腔注射。所有动物分组于术后24 h处死,以损伤段为中心上下取脊髓10 mm和大鼠腔静脉血清2 m L,采用ELISA法检测各组大鼠脊髓组织和血清中TNF-α和IL-6表达水平。结果:(1)单纯损伤组大鼠脊髓组织和血清中TNF-α和IL-6水平均高于假手术组,差异有统计学意义(P<0.05)。(2)柴胡皂苷a 5 mg/kg组、10 mg/kg组、20 mg/kg组大鼠脊髓组织和血清中TNF-α和IL-6水平均低于单纯损伤组,差异有统计学意义(P<0.05)。(3)柴胡皂苷a 10 mg/kg组、20 mg/kg组大鼠脊髓组织中TNF-α和IL-6表达水平低于5 mg/kg组,差异有统计学意义(P<0.05),10 mg/kg组与20 mg/kg组差异无统计学意义(P>0.05)。大鼠血清中TNF-α和IL-6表达水平柴胡皂苷a 20 mg/kg组低于5 mg/kg组,差异有统计学意义(P<0.05),10 mg/kg组与20 mg/kg组差异无统计学意义(P>0.05)。结论:柴胡皂苷a通过影响急性脊髓损伤早期炎症因子的表达减轻继发性免疫炎症反应,从而改善机体免疫功能;10mg/kg组效果优于其他实验组,且该药物可能部分通过脊血屏障。Objective： To investigate the effect of saikosaponina on the expression of tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） in the early stage of acute spinal cord injury in rats. Methods： Thirty SD rats were randomly divided into sham operation group, simple injury group, saikosaponin a 5 mg / kg group, 10 mg / kg group, 20 mg / kg group, respectively. The acute spinal cord injury model was made by modified ,Allen,s heavy blow method. The rats in the injury group were injected with normal saline only, and the drug groups were intraperitoneally injected with the different dosages. All the animals were sacrificed at 24 h after the operation. The spinal cord and the vena cava sera of the injured segment were taken for 10 minutes and 2 minutes, respectively. The levels of TNF-αand IL-6 in the spinal cord tissue and serum were detected by enzyme-linked immunosorbent assay（ELISA）. Results： TNF-αand IL-6 in the spinal cord tissue and serum of the rats in the pure injury group were higher than those in the sham group （P 〈0.05）. The levels of TNF-αand IL-6 in spinal cord tissue and serum of rats treated with saikosaponin a 5 mg / kg, 10 mg / kg and 20 mg / kg were lower than those of the injury group, and the difference was statistically significant （P 〈 0.05）. The expressions of TNF-α and IL-6 in the spinal cord tissue of rats treated with 10 mg / group and 20 mg / kg group the difference were not statistically significant （P〉 0.05）. The levels of TNF-α and IL-6 in serum of rats treated with 10mg / kg and 20 mg/kg were lower than those of 5mg/kg group （P〉 0.05）. Conclusion： Saikosaponina could reduce the secondary immune inflammatory response by affecting the early expression of inflammatory factors to improve the body’s immune function in acute spinal cord injury. The10mg/kg group is superior to the other groups, and the drug may partially pass through the spinal blood barrier.

关 键 词：急性脊髓损伤 柴胡皂苷A 肿瘤坏死因子Α 白介素6 大鼠 

分 类 号：R744[医药卫生—神经病学与精神病学]