低聚木糖联合壳寡糖保护小鼠急性肝损伤及提高脾脏细胞免疫活性的作用  

A study on the Xylo-oligosaccharides in combination with chitooligosaccharides in protecting mice from alcohol-induced liver injury and modulate immune function in mice

作　　者：宋燕华[1] 蔡德雷[1] 夏勇[1] 鹿伟[1] 傅剑云[1] 徐彩菊[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《预防医学》2017年第7期684-688,共5页CHINA PREVENTIVE MEDICINE JOURNAL

摘　　要：目的研究低聚木糖联合壳寡糖对小鼠急性肝损伤及脾细胞免疫活性的调节作用。方法取低聚木糖(0.045~0.26 g/kg)和壳寡糖(0.055~0.32 g/kg)混合物灌胃30 d后,分别以酒清性肝损伤模型和正常小鼠为对照,采用肝匀浆中丙二醛(MDA)、还原型谷胱甘肽(GSH)和三酰甘油(TG)含量测定,肝脏组织病理学检查,脾脏NK细胞活性、淋巴细胞转化能力和抗体生成能力检测,评价低聚木糖协同壳寡糖对急性酒清性肝损伤模型小鼠肝脏的保护作用,以及对正常小鼠脾脏细胞免疫活性的影响。结果急性肝损伤试验结果显示,与空白对照组比较,模型对照组小鼠MDA升高,GSH降低,差异均有统计学意义(P<0.05);各剂量组小鼠MDA水平,低、中剂量组小鼠TG水平,高剂量组小鼠肝脏细胞病理组织学脂肪变性得分均值均低于模型对照组(P<0.05)。免疫活性试验显示,各剂量组和空白对照组小鼠脾脏NK细胞活性比较,差异无统计学意义(P>0.05);与空白对照组比较,中、高剂量组脾淋巴细胞增殖能力和抗体生成能力增强(均P<0.05)。结论在本实验条件下,低聚木糖联合壳寡糖对小鼠急性酒精性肝损伤具有一定的保护作用,可增强小鼠脾细胞免疫活性。Objective To investigate effects of xylo-oligosaccharides with chitooligosaccharides on alcohol-induced liver injury and immune function in mice. Methods Different doses of chitosan oligosaccharide （0.045 g/kg-0.26 g/kgB.W） and xylo-oligosaccaride （0.055 g]kg-0.32 g/kgB.W） were feed to the mice for 30 days. The mice live-injury model was induced by alcohol. MDA, GSH, TG level in liver and T lymphocyte proliferation of spleen cells induced by conA, the antibody-producing cells, and natural killer （NK） activity were detected. Results Compared with the live-injury model group, MDA level of low/middle dose group, TG level of middle dose group and pathological evaluation score of liver steatosis of high dose group in mice liver were decreased because of chitosan oligosaccharide and xylo--oligosaccaride feeding. Compared with the control group, the ability of T lymphocyte proliferation of mouse spleen induced by ConA and the antibody-producing cells were increased in mice of middle and high dose group. The differences had statistical significances （P〈0.05） . Conclusion Under this experimental condition, xylo-oligosaccharides in combination with chitooligosaccharides could protect the mice from alcohol-induced liver injury and enhance immune function in spleen of normal mice synergistically.

关 键 词：低聚木糖 壳寡糖 肝损伤 免疫功能 

分 类 号：R285[医药卫生—中药学]