适量白酒对动脉粥样硬化大鼠血脂及炎性因子的影响  

Effect of moderate drinking on blood lipids and inflammatory factors in rats with atherosclerosis

作　　者：李钰[1] 张欢[1] 饶家权[2] 刘银[1] 王烨[1] 杜礼泉[2] 周玲旭 裴晓方[1] 许欣[1] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]四川省绵阳市丰谷酒业有限责任公司,四川绵阳621000

出　　处：《现代预防医学》2017年第13期2347-2350,共4页Modern Preventive Medicine

摘　　要：目的研究适量白酒对动脉粥样硬化模型大鼠血脂及炎性因子MMP-2、TNF-α及IL-6的影响。方法将160只雄性SD大鼠随机分为空白组和模型组,模型组以高脂饲料联合维生素D3建立动脉粥样硬化模型,12周后将模型组随机分为模型对照组和剂量1、2、3组,剂量组分别以0.9、1.1、2.2 ml/(kg·d)的丰谷48°浓香型白酒灌胃,模型对照组及空白组以2.2 ml/(kg·d)纯水灌胃。12周后股动脉取血测定血脂及血清中基质金属蛋白酶-2(MMP-2)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的含量,取主动脉进行病理学检查。结果给予高脂饲料及维生素D3腹腔注射后,模型组大鼠体重明显下降。主动脉病理切片结果显示,模型对照组可见明显的内膜突出管腔增厚,内皮下大量脂质沉积和泡沫细胞积聚,3个剂量组内膜均出现不同程度的增厚和纤维细胞增生,但程度较模型对照组轻。与空白对照组相比,模型对照组TC、HDL-C、LDL-C、MMP-2、TNF-α、IL-6含量均升高(P<0.05),TG含量降低(P<0.05)。与模型对照组相比,3个剂量组均能明显提高HDL-C水平(P<0.05),剂量2、3组能明显降低MMP-2的水平(P<0.05)。结论适量白酒可以上调HDL-C水平和抑制MMP-2的表达可能在一定程度上具有预防动脉粥样硬化发生发展的作用。Objective To investigate the effects of moderate drinking on the level of the lipids, MMP - 2, TNF - aand IL - 6 in rats with experimental atherosclerosis. Methods 160 male Sprague - Dawley rats were randomly divided into control group and model group. The rats in model group were injected with vitamin D3 and loaded with high -fat diet. After 12 weeks,dividing the model group into a model control group and three dose groups. Rats in the three - dose group were fed with different doses of 48% Luzhou- flavor liquor （0.9/1.1/2.2 ml/（ kg· d）） ,control group and model control group were given saline with the dose of 2.2 ml/ （ kg · d）. After 12 consecutive weeks, lipids, MMP - 2, TNF - α, IL - 6 and aortic pathological changes were tested. Results The body weight of rats decreased after high - fat diet with an injection of vitamin D3. Intragastrically administered Luzhou- flavor liquor for 12 weeks, aortic pathological results showed that aortic was significantly serious with intimal thickening and obvious protrusion to the cavity ,fibrous cap in subcutaneous lipid deposition and foam ceils accumulate in the model control group, three dose groups lining were different degree of thickening and fibre cell proliferation, but the degree was lighter than model control group. Compared with the control group, model control group of serum total cholesterol （TC）, high - density lipoprotein cholesterol （ HDL - C ）, low - density lipoprotein cholesterol （ LDL - C ）, matrix metalloproteinase - 2 （ MMP - 2）, tumor necrosis factor alpha （TNF -α） ,interleukin 6 （ IL - 6） content were higher （P 〈 0. 05）, triglyceride （TG） were decreased （ P 〈 0. 05 ）. Compared with model control group, three dose groups all could significantly improve the level of HDL- C （P 〈 0.05 ）, a dose of 2,3 group could obviously reduce the level of MMP- 2（P 〈 0.05 ）. Conclusion Moderate drinking can reduce the formation of atherosclerosis, to a certain extent, prevent occurrence and development

关 键 词：适量饮酒 动脉粥样硬化 血清生化指标 炎症因子 主动脉 

分 类 号：R15[医药卫生—营养与食品卫生学]