复方楂金颗粒剂对非酒精性脂肪性肝炎大鼠Toll样受体4的影响  

Effect of Complex Prescription Zhajin Drug Granules on Expression of Toll-like Receptor 4 in Rats with NASH

作　　者：向晶[1] 施军平[2] 陈海君[1] 邵兴[1] 

机构地区：[1]金华市中心医院,浙江金华321000 [2]杭州师范大学附属医院,浙江杭州310000

出　　处：《中华中医药学刊》2017年第7期1719-1723,I0010,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81070316)

摘　　要：目的:探讨复方楂金颗粒剂(CPZD)对非酒精性脂肪性肝炎(NASH)大鼠Toll样受体4(TLR4)、髓样分化因子88(My D88)表达的影响。方法:雄性SD大鼠分为6组,正常组正常饮食,模型组、治疗组给予高脂饲料喂养10周诱发脂肪性肝炎后分别以生理盐水、易善复、复方楂金颗粒剂高、中、低剂量灌胃,每日1次,共10周。处死大鼠,分别进行如下检测:(1)计算肝指数;(2)HE染色,光镜下观察各组大鼠肝组织的病理改变;(3)用免疫组织化学染色和RT-PCR法检测大鼠肝组织TLR4、My D88蛋白和mRNA的表达;结果:模型组NAFLD活动度积分(NAS)明显高于正常组;模型组的TLR4、My D88蛋白和mRNA表达均高于正常组的TLR4、My D88蛋白和mRNA表达(P<0.01,P<0.05);易善复组、CPZD高、中、低剂量组TLR4、My D88蛋白和mRNA表达均高于正常组而低于模型组,但只有CPZD中剂量组TLR4、My D88蛋白和mRNA表达相对于模型组有统计学意义(P<0.05)。结论:复方楂金颗粒剂能抑制脂质在大鼠肝脏内沉积,具有防治NASH作用;复方楂金颗粒剂能明显减少NASH大鼠肝组织TLR4和My D88基因表达,通过My D88依赖途径减轻肝脏炎症。Objectives：To validate the effect of Zhajin Drug Granules（CPZD） on TLR4 and MyD88 expressions of rats in order to approach the possible mechanism of CPZI） against NASH. Methods： SD rats were randomly divided into six groups：control group（ normal diet）, model group and treatment group which were given high fat diet to induce steatohepa- titis for10 weeks,respectively. Rats in model group were treated with normal saline and those in treatment group was fed high,medium and low doses of CPZD respectively. And then rats were sacrificed. The following tests were performed：he- patic lipid contents, histopathological changes in the liver and hepatic TLR4 mRNA expression leves. Results： Model group＇ s NAS was significantly higher than the normal group＇ s. TLR4, MyD88 protein and mRNA expressions in model group were higher than normal group＇s TLRd, MyD88 protein and mRNA expressions（P 〈 0. 01,P 〈 0. 05 ）. Essentiale group, CPZD high, medium and low dose groups＇ TLR4, MyD88 protein and mRNA expression were higher than normal but lower than the model group＇ s, but only CPZD medium dose group＇ s TLR4, MyD88 protein and mRNA expressions were relative to model group＇ s. It was statistically significant（P 〈 0. 05）. Conclusions： CPZD can repress fat deposition in the liver of rats and can prevent and treat NASH. It also can markedly depress the gene expressions of TLR4 and MyD88 and reduce the inflammation of the liver through the MyD88 - dependent pathway.

关 键 词：脂肪肝 TOLL样受体4 髓样分化因子88 

分 类 号：R575.1[医药卫生—消化系统]