新型保加利亚乳杆菌微胶囊的稳定性  

Stability of the new type of L. Bulgaricus microcapsule

作　　者：周奕先 李峰[1,2] 王倍[1] 陈锦填 黄莹[1] 潘昕[1] 李革 陈航平 冯敏[1] 吴传斌[1] 

机构地区：[1]中山大学药学院,广东广州510006 [2]广州新济药业科技有限公司,广东广州510006

出　　处：《药学学报》2017年第7期1165-1169,共5页Acta Pharmaceutica Sinica

基　　金：国家高技术研究发展技术(863计划)资助项目(2014AA022205)

摘　　要：为提高保加利亚乳杆菌制剂的长期稳定性和对不良环境的耐受能力,本文制备了一种新型的保加利亚乳杆菌微胶囊。保加利亚乳杆菌微胶囊最优工艺流程和条件为:将保加利亚乳杆菌与3%海藻酸钠溶液混合,其中菌悬液浓度约为1×10~9 cfu·mL^(-1)(cfu:colony-forming units),通过点胶机滴至灭菌的2%CaCl_2溶液,固化30 min后冷冻干燥得微胶囊,以提高保加利亚乳杆菌的稳定性和存活率。所制得的微胶囊颗粒圆整、粒径均一、囊壁结构保持完整。对冻干菌粉及微胶囊进行耐酸性能、耐高温性能、耐高湿性能和长期稳定性等研究,结果显示微囊化技术可以明显改善保加利亚乳杆菌的稳定性以及对不良环境的耐受能力。A new type of L. Bulgaricus microcapsule was prepared to improve stability and resistance of L. Bulgaricus probiotics in harsh environments. An optimal method of preparation of L. Bulgaricusmicrocapsule is as follow. L. Bulgaricus was mixed with 3% alginate solution. The concentration of bacterial suspension was 1×10~9 cfu·mL^（-1）. The mixture was microencapsulated by extrusion into 2% CaCl_2 solution with a dispensing equipment. After 30 min solidification, the gel beads were lyophilized to obtain L. Bulgaricus microcapsules. The microencapsulation technology was aimed to improve the stability and survival rate of L. Bulgaricus. The microcapsule was spherical with uniform particle size and intact structure. The tolerance of acid, high temperature, high humidity and the long-term stability of freeze-dried powder and microcapsule were evaluated. The results indicated that microencapsulation technic could greatly improve stability and resistance of L. Bulgaricus probiotics in harsh environments.

关 键 词：益生菌 乳酸菌 胶囊 药物稳定性 

分 类 号：R943[医药卫生—药剂学]