基于在线参数动态调控的双酶法生产葡萄糖酸钠新工艺  

A novel approach for sodium gluconate production using two-enzyme method by on-line parameter monitoring and dynamic regulation

作　　者：苏立宇 赵伟 杭海峰[1] 储炬[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]山东福洋生物科技有限公司,山东德州253100

出　　处：《食品与发酵工业》2017年第6期21-26,共6页Food and Fermentation Industries

基　　金：国家重点基础研究发展计划(2013CB733600);国家高技术研究发展计划(2015AA021005)

摘　　要：采用葡萄糖酸钠生产过程中在线参数氧消耗速率(OUR)和溶氧(DO)作为调控参数,开发出阶段性实时动态补加葡萄糖氧化酶的双酶法生产葡萄糖酸钠新型工艺,酶催化过程中葡萄糖氧化酶酶活始终不是限制因素。50 L反应器初始葡萄糖浓度在330 g/L条件下,能够有效将生产时间从原始工艺的17.0 h缩短至11.6 h,得率由1.167(g/g)提高至1.176(g/g),最终消耗的葡萄糖氧化酶的量也由2 100 U/g葡萄糖缩减到1 444 U/g葡萄糖。与生物发酵相比,改进后的酶法工艺也更具优越性,生产时间缩短了42.85%,得率提升了4.91%。改进后的双酶法生产葡萄糖酸钠新工艺具有更广阔的市场应用前景。In this paper, much attention was paid on the on-line parameters oxygen uptake rate （OUR） and dis- solved oxygen （DO） monitoring and control. Based on the changing trends of these parameters, a novel approach of sodium gluconate production was proposed by stepwise addition of glucose oxidase solution dynamically, and the activ- ity of glucose oxidase was kept at an unlimited level during the whole production period. The results showed that in the 50 L reactor with 330 g/L initial glucose concentration, the production time was shortened form 17.0 h to 11.6 h, the yield was enhanced from 1. 167 g/g to 1. 176 g/g and the overall dosage of used glucose oxidase declined from 2 100 U/g glucc,se to 1 444 U/g glucose respectively. Compared to the biological fermentation, the novel direct enzy- matic reaction displayed significant advantages. The production time was cut down by 42.85% and the yield was 4. 91% higher than the traditional biological fermentation. The improved direct enzymatic production had more potential value in the further industrial application.

关 键 词：葡萄糖氧化酶 葡萄糖酸钠 在线参数 工艺优化 

分 类 号：TQ261.1[化学工程—有机化工]