EP2受体和FP受体对奶牛输卵管上皮细胞输卵管蛋白的影响  

EP2 and FP receptors mediate oviductin synthesis and secretion in bovine oviductal epithelial cells

作　　者：张楠[1] 张莹[1] 曹金山[1] ZHANG Nan ZHANG Ying CAO Jin-shan(Collegeof Veterinary Medicine,InnerMongolia Agricultural University, Hohh ot 010018, China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2017年第7期1394-1400,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(3160490)

摘　　要：采用免疫荧光法,使用角蛋白鉴定奶牛输卵管上皮细胞;采用荧光定量PCR法检测奶牛输卵管上皮细胞输卵管蛋白mRNA的表达;采用Wsetern blot法检测了奶牛输卵管上皮细胞输卵管蛋白的表达。结果显示:加入butaprost(EP2受体激动剂,10^(-6) mol/L)能促进输卵管蛋白的表达,加入fluprostenol(FP受体激动剂,10^(-6) mol/L)能抑制输卵管蛋白的表达。结果表明:PGE2在奶牛输卵管上通过激活EP2受体上调输卵管蛋白的表达,PGF2α通过激活FP受体下调输卵管蛋白的表达。In this study, bovine oviductal epithelial cells were identified by immunof｜uoresence with keratin. Messenger RNA expression of oviductal proteins was investigated using RT-PCR, and protein expression was analysed using Western blot analysis. The results indicated that after butap- rost（EP2 receptor agonists, 10^-6 mol/L） treatment,the expression of oviductin was significantly promoted and reached a peak at 8 h compared with the blank control group. After adding the fluprostenol（FP receptor agonist, 10^-6 mol/L）, the expression of oviductin decreased. The experi- mental results showed that PGE2 could up regulate the expression of oviductin by EP2 receptor, but PGF2α could down regulate the expression of oviductin by FP receptor.

关 键 词：PGE2 PGF2Α EP2受体 FP受体 输卵管 输卵管蛋白 

分 类 号：S814.4[农业科学—畜牧学]