FasL的特异性miRNA阻断舌鳞癌细胞免疫逃逸的研究  

FasL-specific microRNA in blocking immune escape of tongue squamous cell carcinoma

作　　者：吴棪 徐艳雪 韩曈曈 陈乔尔[1,2] 朱友明[1,2] 

机构地区：[1]安徽医科大学口腔医学院,合肥230032 [2]安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,合肥230032

出　　处：《安徽医科大学学报》2017年第8期1115-1119,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1508085MH155)

摘　　要：目的研究特异性微小RNA(miR)-590对舌鳞癌细胞SCC-3 Fas配体(FasL)的表达的影响。方法将合成的miR-590 mimics或miR-590抑制剂转染至SCC-3细胞。通过RT-PCR和Western blot法检测SCC-3中Fas L信使RNA(mRNA)和蛋白的表达;将转染后的SCC-3细胞在顺铂(DDP)5 mg/L处理下,在不同时间段通过MTT法绘制细胞生长曲线,并通过针对Fas L的miR-590的抑制剂从反面验证miR-590的作用。结果 miR-590可以在mRNA和蛋白水平上降低SCC-3细胞Fas L的表达;转染miR-590后SCC-3细胞对于DDP的耐药性降低。结论 miR-590可以有效抑制SCC-3细胞Fas L的表达,降低SCC-3的DDP的耐药性,从而可能降低和减少其对免疫活性细胞的凋亡诱导和杀伤作用,从而阻断肿瘤细胞的免疫逃逸,为舌鳞癌的生物治疗提供了新的靶点。Objective To investigate the effect of miR-590 on the expression of FasL in SCC-3 cells. Methods The synthetic miR-590 mimics or miR-590 inhibitor was transfected into SCC-3 cells. The mRNA and protein ex-pression of FasL mRNA and protein in SCC-3 were detected by RT-PCR and Western blot respecitively. The trans-fected SCC-3 cells were treated with Cisplatin （ DDP） 5 mg/L and the cell growth curves were drawn at different time points by MTT assay, and the effect of miR-590 was verified from the negative side by the inhibitor of miR-590 against FasL. Results MiR-590 decreased the expression of FasL on SCC-3 cells at mRNA and protein levels; Re-sistance to DDP was decreased in SCC-3 cells after miR-590 transfection. Conclusion MiR-590 can effectively in-hibit the expression of FasL in SCC-3 cells and decrease the resistance of SCC-3 to DDP, which may reduce the ap-optosis-inducing and killing effect of immunocompetent cells, blocking tumor immune escape, providing a new target for the treatment of tongue squamous cell carcinoma.

关 键 词：FASL miR-590 舌鳞癌细胞 

分 类 号：R739.8[医药卫生—肿瘤] R730.51[医药卫生—临床医学]