HIF-1α基因转染人胃腺癌SGC7901细胞对裸鼠移植瘤的影响及机制  

Effect and possible mechanism of HIF-1α gene transfection of human gastric adenoma SGC7901 xenografts in nude mice

作　　者：汪晓庆[1] 曹威[2] 宋先兵[1] 潘献柱[1] 吴义春[1] 陈晓宇[2] 

机构地区：[1]安徽医学高等专科学校病理学教研室,合肥230601 [2]安徽医科大学组胚教研室,合肥230032

出　　处：《安徽医科大学学报》2017年第8期1128-1132,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究重点项目(编号:KJ2015A359);国家自然科学基金资助项目(编号:81373421)

摘　　要：目的探讨低氧诱导因子(HIF)-1α基因转染人胃腺癌SGC7901对裸鼠移植瘤血管生成及Eph A2表达的影响及其可能机制。方法采用HIF-1α基因重组质粒p GCsish HIF-1α,转染胃腺癌SGC7901细胞并接种裸鼠(SGC7901/shRNA,实验组),建立裸鼠皮下人胃腺癌移植瘤模型,动态观测裸鼠肿瘤体积和质量;设SGC7901(未转染组)、转染空质粒的SGC7901(SGC7901/Neo,空载体组)为对照。观察移植瘤生长情况,8周后,获取移植瘤模型瘤组织,称取湿重,免疫组化SP法检测移植瘤组织的微血管密度,Western blot检测上皮细胞激酶A2(Eph A2)表达。结果与未转染组比较,空载体组瘤体积、质量改变不明显,HIF-1α转染的实验组的瘤体积、质量明显降低;HIF-1α转染的实验组诱导血管增生、形成血管生成拟态和Eph A2蛋白表达较未转染组组、空载体组明显降低(P<0.05,P<0.01)。结论 HIF-1α基因转染可明显抑制裸鼠人胃腺癌移植瘤的生长,该作用可能与其抑制肿瘤血管新生、形成血管拟态及Eph A2蛋白表达有关。Objective To study the effect of hypoxia inducing factorl alpha（ HIF-la） gene transfection of human gastric adenocarcinoma on nude mice transplantation tumor and its possible mechanism. Methods Set SGC7901 （untransfected group） , empty plasmid transfection SGC7901 （ SGC7901/Neo, blank load transfection group） for comparison, gene recombinant plasmid pGCsi-shHIF-la, transfection gastric carcinoma SGC7901 cells（ SGC7901/ shRNA, experimental group） inoculated nude mice, to establishanude mice subcutaneous transplantation tumor of gastric adenocarcinoma model, dynamic observation of nude mice tumor volume and body quality. After eightweeks, to get tumor tissue transplantation tumor model, weigh wet weight, microvascular density of transplanted tumor tis-sue was detected by immunohistochemical SP method, epithelial cell kinase A2 （ EphA2 ） expression was detected by Western blot. Results Compared with untransfectedand blank load transfection group, HIF-la gene transfection in experimental group tumors had significantly lower in tumor volume, quality and vascular proliferation, EphA2 protein expression significantly lower than that in untransfected and blank load transfection group （ P 〈 0. 05, P 〈 0. 01 ） . Conclusion HIF-la gene transfection can inhibit the growth of nude mouse transplantation tumor of gastric adenocarcinoma significantly, which may relateits inhibiting tumor angiogenesis and EphA2 protein expression.

关 键 词：胃腺癌 裸鼠 低氧诱导因子1Α 基因转染 血管生成 

分 类 号：R977.3[医药卫生—药品]