EGFR基因knockdown对肝癌细胞MHCC-97H侵袭迁移能力的影响  

Effect of EGFR gene knockdown on invasion and migration of hepatocellular carcinoma MHCC-97H cell line

作　　者：张传海[1] 桂阳[1] 郭凤林[2] 马金良[1] 余继海[1] 

机构地区：[1]安徽医科大学附属省立医院普外科,合肥230001 [2]安徽医科大学附属省立医院麻醉科,合肥230001

出　　处：《安徽医科大学学报》2017年第8期1133-1137,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金项目资助项目(编号:1608085MH198)

摘　　要：目的探讨RNA干扰表皮生长因子受体(EGFR)基因对肝癌细胞株MHCC-97H侵袭和迁移能力的影响。方法构建EGFR基因shRNA,使用Lipofectamine 2000转染MHCC-97H细胞,Real-time PCR和Western blot检测EGFR和pEGFR的表达;细胞划痕、Transwell检测MHCC-97H细胞侵袭和迁移能力的变化。结果与正常组相比,转染EGFR shRNA组EGFR和p-EGFR均显著降低(P<0.05);同时,细胞的侵袭、迁移能力均明显减弱(P<0.05)。结论干扰EGFR可降低MHCC-97H细胞侵袭、迁移能力,降低其远处转移能力。Objective To investigate the effect of RNA interference on the invasion and migration of hepatocellular carcinoma cell by epidermal growth factor receptor gene. Methods The expression of EGFR and p - EGFR was de-tected by Real-time PCR and Western blot. Cell scratch and Transwell were used to detect the invasion and migra-tion of MHCC-97H cells. Lipofectamine 2000 was used to transfect MHCC-97H cells. Results EGFR and p-EG- FR were significantly decreased in EGFR siRNA group compared with the normal group （P 〈0. 05） . Meanwhile, the invasion and migration of EGFR siRNA group were significantly decreased （ P 〈 0. 05 ） . Conclusion Interfer-ing with EGFR can reduce the ability of invasion and migration of MHCC-97H cells and reduce the distant metasta-sis ability of MHCC-97H cells.

关 键 词：EGFR p-EGFR MHCC-97细胞 RNA干扰 侵袭迁移能力 

分 类 号：R392.11[医药卫生—免疫学] R329.25[医药卫生—基础医学]