沉默Survivin基因对人头颈鳞癌细胞周期以及细胞凋亡的影响  

Investigate the effect of Survivin gene silencing on cell apoptosis in human head and neck squamous cell carcinoma

作　　者：王娟[1] 张曙光[1] 孙颖[1] 刘明[1] 

机构地区：[1]唐山市中医医院病理科,河北唐山063000

出　　处：《临床和实验医学杂志》2017年第13期1288-1291,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的采用基因沉默手段沉默人头颈鳞癌细胞中Survivin基因的表达,进而探讨Survivin基因沉默对人头颈鳞癌细胞凋亡的影响。方法采集人头颈鳞癌PCI-37B细胞作为观察对象,根据细胞处理方式的差异将其分为观察组、阴性对照组、空白对照组。其中观察组采用基因沉默手段对PCI-37B细胞进行处理,将Sruvivin-SiRNA进行细胞转到入PCI-37B。阴性对照组选用FAM-SiRNA-Mate复合物。空白对照组则应用未进行转染处理的正常细胞。采用流式细胞仪检测PCI-37B细胞凋亡情况,观察细胞周期的变化,并与阴性对照组、空白对照组进行比较。结果Survivin基因沉默处理后,人头颈鳞癌细胞PCI-37B细胞凋亡率明显升高,与阴性对照组、空白对照组比较差异存在显著,并且阴性对照组与空白对照组比较无显著差异(P>0.05);细胞周期变化观察结果显示,Survivin基因沉默能够对肿瘤细胞周期产生影响,降低鳞癌细胞在S期的比率,与阴性对照组、空白对照组比较,差异具有统计学意义(P<0.05)。结论应用基因沉默手段沉默Survivin基因的表达可有效诱导人头颈鳞癌细胞PCI-37B细胞的凋亡活动,对抑制肿瘤细胞的生长以及浸润具有重要作用,临床可以此作为切入点研究、制定人头颈鳞癌的靶向治疗方案。Objective Expression of Survivin gene silencing of human head and neck squamous cell carcinoma gene silencing,and to explore the effect of Survivin gene silencing on apoptosis of human head and neck squamous cell carcinoma.Methods The acquisition of head and neck squamous cell carcinoma cell line PCI-37B cells were selected as the observation object,by means of gene silence treated PCI-37B cells,Sruvivin-SiRNA cells go into PCI-37B,after the detection of PCI-37B cell apoptosis by flow cytometry,the changes of cell cycle were observed,and compared with the negative control group,Su analysis Effect of rvivin gene silencing on human head and neck squamous cell carcinoma PCI-37B cell apoptosis.Results Survivin gene silencing after treatment,apoptosis rate of head and neck squamous cell carcinoma PCI-37B cell increased,there was significant difference,and the difference with the negative control group was statistically significant （P＆lt;0.05）.The change of cell cycle observation showed that Survivin gene silencing could affect the tumor cell cycle,decrease squamous cell percentage in S phase,there were significant differences （P＆lt;0.05）.Conclusion Survivin gene has an important effect on the apoptosis of human head and neck squamous cell carcinoma.The apoptosis activity by gene expression silencing Survivin gene silencing can effectively induce human head and neck squamous cell carcinoma cell line PCI-37B cells,it plays an important role in inhibiting tumor cell growth and invasion,which can be used as clinical research starting point,and to develop head and neck squamous cell carcinoma targeting treatment plan.

关 键 词：人头颈鳞癌 SURVIVIN基因 基因沉默 细胞凋亡 影响 

分 类 号：R739.91[医药卫生—肿瘤]