免疫组化检测GPC-3与CK-19在肝细胞癌诊断中的应用价值  

The application value of GPC-3 and CK-19 in the diagnosis of hepatocellular carcinoma

作　　者：王娟[1] 张曙光[1] 孙颖[1] 刘明[1] 

机构地区：[1]唐山市中医医院病理科,河北唐山063000

出　　处：《临床和实验医学杂志》2017年第14期1398-1401,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨磷脂酰肌醇蛋白多糖-3(GPC-3)与细胞角蛋白-19(CK-19)免疫组化检测项目在肝细胞癌临床诊断及鉴别诊断中的应用价值。方法选取2012年1月至2016年1月收集的肝癌细胞标本98例作为研究对象。其中肝细胞癌71例,肝内胆管细胞癌18例,肝细胞癌合并肝内胆管细胞癌9例。对所有标本进行免疫组化检测,观察GPC-3与CK-19在肝细胞癌、肝内胆管细胞癌以及肝细胞癌合并肝内胆管细胞癌组织中的分布及表达情况。结果肝细胞癌、肝细胞癌合并肝内胆管细胞癌中GPC-3阳性率均明显高于肝内胆管细胞癌,差异有统计学意义(P<0.05)。而肝内胆管细胞癌、肝细胞癌并肝内胆管细胞癌标本中CK-19阳性率明显高于肝细胞癌(P<0.05)。并且中低分化的肝细胞癌标本中GPC-3的阳性率明显高于高分化肝细胞癌(P<0.05)。中低分化肝癌标本中CK-19的阳性率明显高于高分化肝癌标本,差异具有统计学意义(P<0.05)。结论免疫组化检测组织内GPC-3以及CK-19的表达情况可提升临床对肝细胞癌、肝内胆管细胞癌、肝细胞癌合并肝内胆管细胞癌诊断的特异性与敏感性。GPC-3联合CK-19免疫组化检测对诊断及鉴别诊断肝细胞癌具有重要的临床应用价值。Objective To investigate the effect of phosphatidylinositol protein polysaccharide-3 （GPC-3） and cytokeratin-19 （CK-19） application value of immunohistochemical detection project in clinical diagnosis and differential diagnosis of hepatocellular carcinoma.Methods From January 2012 to August 2016 pathological specimens of 98 cases of hepatocellular carcinoma were collected, including 71 cases of hepatocellular carcinoma.98 cases of hepatocellular carcinomas, 18 cases of intrahepatic cholangiocarcinoma and hepatocellular carcinoma complicated with intrahepatic cholangiocarcinoma in 9 cases.The distribution and expression of GPC-3 and CK-19, GPC-3 and CK-19 in hepatocellular carcinoma, intrahepatic cholangiocarcinoma and hepatocellular carcinoma complicated with intrahepatic bile duct carcinoma were detected, and the application value of GPC-3 and CK-19 detection in the diagnosis and differential diagnosis of hepatocellular carcinoma were analyzed.Results GPC-3 positive rate in hepatocellular carcinomas and hepatocellular carcinoma complicated with intrahepatic cholangiocarcinoma were significantly higher than that of intrahepatic cholangiocarcinoma （P〈0.05）.CK-19 positive rate in intrahepatic cholangiocarcinoma and hepatocellular carcinoma complicated with intrahepatic cholangiocarcinoma were significantly higher than that of hepatocellular carcinomas （P〈0.05）.GPC-3 and CK-19 positive rates in moderately or poorly differentiated hepatocellular carcinomas were higher than that of high differentiated hepatocellular carcinomas （P〈0.05）.Conclusion The immunohistochemistry detection of the expression of GPC-3 and CK-19 in tissues can improve the clinical diagnosis sensitivity and specificity of hepatocellular carcinoma, intrahepatic cholangiocarcinoma, hepatocellular carcinoma complicated with intrahepatic cholangiocarcinoma.GPC-3 combined with CK-19 immunohistochemical detection has important clinical application value for the diagnosis and differential diagnosis of hepatocellular carcinoma.

关 键 词：肝细胞癌 肝内胆管细胞癌 磷脂酰肌醇蛋白多糖-3 细胞角蛋白-19 免疫组化 临床诊断 

分 类 号：R730.43[医药卫生—肿瘤] R735.7[医药卫生—临床医学]