紫檀芪调节转移相关基因1表达抑制肝癌生长的机制  

Mechanism of pterostilbene inhibiting growth of hepatocellular carcinoma by regulating metastasis-associated protein 1 expression

作　　者：尹建勋[1] 王玲[2] 孙华朋[2] 廖晓锋[2] 李锟[1] 

机构地区：[1]武汉大学中南医院肝胆胰外科,430070 [2]襄阳市中心医院普外科,441021

出　　处：《中华实验外科杂志》2017年第7期1108-1110,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨紫檀芪（PTER）调节转移相关基因1（MTA1）表达抑制肝癌生长的机制。方法应用实时定量聚合酶链反应（Real-time PCR）法检测MTA1基因在78例肝癌组织以及相应的癌旁组织中的表达，分析其表达与肝癌临床病理特征的关系。同时PTER作用于SSMC-7721细胞24 h，Western blot及噻唑蓝（MTT）检测其蛋白表达和细胞活力。结果（1）肝癌组织及癌旁组织中MTA1的阳性表达率分别为92.3%（72/78）和84.6%（66/78，P=0.000）。MTA1在肝癌TNM分期中Ⅲ～Ⅳ期癌组织中的表达明显高于肝癌分期Ⅰ～Ⅱ期癌组织；MTA1在肝癌组织的表达水平与肝内转移、门静脉浸润明显相关。（2）PTER解析MTA1/组蛋白去乙酰化酶蛋白复合物，PTER处理后HCC的Acp53/p53为0.64，肝癌细胞活性与PTER剂量呈反比（P=0.000）。（3） MTA1的表达与肝癌的增殖、转移和临床分期有关；与肝癌TMN分期呈正相关。结论PTER通过抑制MTA1的表达来阻滞肝癌细胞的生长和进展。Objective To explore the action mechanism of pterostilbene inhibiting growth of hepatocellular carcinoma （HCC） by regulating metastasis-associated protein 1 （MTA1） expression.Methods The expression of MTA1 mRNA was detected by real-time quantitative polymerase chain reaction （Real-time PCR） in 78 cases of human HCC tissues and the counterparts from 78 cases of paracarcinoma tissues. The relationship between the expression of MTA1 with clinicopathological features of HCC was evaluated. The SSMC-7721 cells were cultured in the presence of pterostilbene for 24 h. The cell viability was measured by methyl thiazol tetrazolium （MTT） assay, and the protein expression of MTA1 was detected by Western blotting.Results The expression of MTA1 was significantly higher in HCC tissues than in matched paracarcinoma tissues. The expression of MTA1 was significantly higher in TMN Ⅲ-Ⅳ HCC tissues than in TMN Ⅰ-Ⅱ HCC tissues （P=0.000）. The expression level of MTA1 in HCC tissues was related to intrahepatic metastasis and portal vein infiltration. There was down-regulation of MTA1, histone deacetylase （HDAC）1 and HDAC2 expression in pterostilbene-treated SSMC-7721 cells compared to vehicle-treated cells. The ratio of Acp53/total p53 was higher in HCC cells after treatment. The cell viability was decreased with increasing pterostilbene dose （P=0.000）.Conclusion The expression of MTA1 may relate to the proliferation, metastasis and clinical stages of HCC. The expression of MTA1 in HCC tissues may be positively correlated with the TMN stages.

关 键 词：转移相关基因1 紫檀芪 癌 肝细胞 

分 类 号：R735.7[医药卫生—肿瘤]