丰富环境对缺血缺氧脑损伤新生大鼠海马脑源性神经营养因子表达及细胞凋亡的影响  

Effects of enriched environment intervention on brain-derived neurotrophic factor expression and apoptosis in hippocampus of hypoxic ischemic brain damaged neonatal rats

作　　者：宋名杨 朱路文[1] 叶涛[2] 姜云飞[1] 赵彬[1] SONG Mingyang ZHU Luwen YE Tao et al(The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin,150001)

机构地区：[1]黑龙江中医药大学附属第二医院,哈尔滨150001 [2]黑龙江中医药大学

出　　处：《中国康复医学杂志》2017年第7期750-755,共6页Chinese Journal of Rehabilitation Medicine

基　　金：黑龙江省高校科技创新团队建设计划项目(2013TD007);黑龙江中医药大学科研基金项目(201506)

摘　　要：目的:探讨丰富环境对缺血缺氧脑损伤(HIBD)新生大鼠海马脑源性神经营养因子(BDNF)表达及细胞凋亡的影响。方法:采用随机数字表法将36只7日龄新生大鼠分为假手术组、模型组及丰富环境组,各组又分为术后7d、28d两个亚组(n=6)。采用Rice-Vannucci经典方法建立新生大鼠HIBD模型。模型组及假手术组不予任何干预,丰富环境组予以早期抚触及丰富环境刺激治疗。术后7d、28d采用水迷宫检测方法评估大鼠学习记忆能力,免疫组化法检测大鼠海马CA1区BDNF的表达,TUNEL法检测海马CA1区细胞凋亡。结果:术后7d、28d,与模型组比较,丰富环境组逃避潜伏期均缩短(P<0.05),穿越平台次数增多(P<0.05),BDNF表达升高(P<0.05),TUNEL阳性细胞数降低(P<0.05)。结论:丰富环境能改善HIBD新生大鼠学习记忆能力,其机制可能与上调海马区BDNF的表达及抑制细胞凋亡有关。Objective： To investigate the effect of enriched environment intervention on brain-derived neurotrophic factor expression and apoptosis in hippocampus of hypoxic-ischemic brain damaged（HIBO） neonatal rats. Method： Thirty-six male 7-day-old rats were randomly divided into sham operation group, model group, enriched environment group, each group was further divided into 7 days and 28 days subgroups （n=6）. HIBD model of neonatal rats was established according to Rice-Vannucci classical methods. The model group and the sham operation group accepted no treatment. Enriched enriched environment group was treated with early touch and enriched environment stimulation. 7 days and 28 days after operation, the learning and memory ability was evaluated with the water maze detection method, the expression levels of BDNF were detected by immuno- histochemical method. The apoptosis was detected by TUNEL method. Result： Compared with the model group, the hidden platform escape latency period （EL） was significantly shortened （P 〈 0.05） in enriched environment group 7 days and 28 days after operation, the cross-platform number within 2 minutes was significantly increased（P〈 0.05）,the expression of BDNF increased （P〈 0.05）,TUNEL positive cell number decreased （P 〈 0.05）. Conclusion： Enriched environment can improve the learning and memory ability in hypoxic-ischemic brain damage neonatal rat, which may be related to the up regulation of BDNF expression and anti-apoptosis in hippocampus.

关 键 词：丰富环境 缺血缺氧性脑损伤 脑源性神经营养因子 凋亡 

分 类 号：R742[医药卫生—神经病学与精神病学]