丰富环境对脑缺血大鼠行为学恢复及神经丝蛋白、突触素表达的影响  

The influence of enriched environment on behavioral recovery and NF,SYN expression after focal ischemia in rats

作　　者：薛丽[1] 廖维靖[1] 蔺俊斌[2] 章鑫[2] 陈修平[2] 徐换 XUE Li LIAO Weijing LIN Junbin et al(Dept. of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University, Hubei, 430071)

机构地区：[1]武汉大学中南医院康复医学科武汉大学脑血管病研究中心,武汉430071 [2]武汉大学中南医院康复医学科

出　　处：《中国康复医学杂志》2017年第7期763-767,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(303163421)

摘　　要：目的:研究"形神共养"对脑缺血大鼠行为学恢复及神经丝蛋白(NF)、突触素(SYN)表达的影响。方法:制作线栓法大鼠脑缺血模型(MCAO),随机分为"形养"组、"神养"组、"形神共养"组、"标准养"组及假手术组。术后第3、10、17、24、30天进行平衡木行走试验及倾斜板试验,干预结束后进行免疫组织化学检测大鼠梗死周围皮质及海马区NF、SYN的表达。结果:平衡木行走试验和倾斜板试验:三个治疗组大鼠评分均优于"标准养"组(P<0.05)。免疫组化检测:"形养"、"神养"、"形神共养"组大鼠梗死周围皮质及海马区NF、SYN表达均高于标准养组,"形神共养"组表达高于其他两组(P<0.05)。结论:"形神共养"的丰富生存环境可促进脑缺血大鼠行为学恢复,其机制可能与促进NF及SYN的表达有关。Objective： To study the effect of ＂both physique and spirit＂ enriched environment on behavioral recovery and expression of NF and SYN. Method： Established the rat model of MCAO, then divided the rats into ＂physique regimen＂ group, ＂spirit regimen＂ group, ＂physique and spirit regimen＂ group, standard group and sham-operated group. The beam-walking test and inclined plane test were used to evaluate the functional recovery at day 3, 10, 17, 24 and 30 after MCAO. NF and SYN were immunohistochemistry stained to detect the expression in peri-infarct and hippocam- pus area after intervention. Result： Rats in three treated groups performed better than in standard group（P〈 0.05） in the beam-walking test and inclined plane test （P〈 0.05）. The expression of NF and SYN in peri-infarct and hippocampus area of rats in three treated groups were significantly higher than in standard group（P〈 0.05）.Moreover, the rats in ＂physique and spirit＂ enriched environment group had the highest expression than the other two groups（P 〈 0.05）. Conclusion： ＂Physique and spirit regimen＂ with enriched environment can promote the functional recovery, which may be associated with the enhanced expression of NF and SYN.

关 键 词：形神共养 丰富生存环境 脑缺血再灌注 神经丝蛋白 突触素 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R493[医药卫生—临床医学]