分子生态学技术用于反硝化微生物群落研究的进展  

Advances in Molecular Ecology Technologies for the Study of Denitrifier Communities

作　　者：张盛博[1] 何小娟[1] 吴海露[1] 王欣泽[1] ZHANG Shengbo HE Xiaojuan WU Hailu WANG Xinze(School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, Chin)

机构地区：[1]上海交通大学环境科学与工程学院,上海200240

出　　处：《环境科学与技术》2017年第6期120-126,共7页Environmental Science & Technology

基　　金：国家重大科技专项(2012ZX07105003)

摘　　要：反硝化微生物是地球氮循环的主要参与者,利用反硝化酶的编码基因,结合现代分子生态学技术,已成为研究环境中反硝化微生物的主要方法。该文介绍了传统的测序技术和新兴高通量测序技术的优缺点,综述了运用分子生态学技术对反硝化微生物开展研究的情况,也对未来分子生态学技术及其在反硝化微生物识别和反硝化作用机理方面的研究进行了展望。Denitrifiers play a dominant role in the earth＇s nitrogen cycle, the modern molecular ecology technology using the encoding gene of the enzyme has become the main method of the denitrifiers in the environment. The advantages and disadvantages of traditional sequencing technology and emerging high throughput sequencing technology are introduced in this paper. The research on the denitrifiers by molecular ecology technology is reviewed, and the future research on molecular ecology technology and its applications in denitrifiers are also discussed.

关 键 词：反硝化微生物 功能基因 分子生态学 

分 类 号：X172[环境科学与工程—环境科学]