补骨脂-肉豆蔻药对含药血清指纹图谱研究  

Serum fingerprint of drug-couple Psoralea corylifolia-Myristica fragrants

作　　者：高家荣[1] 徐双枝 韩燕全[1] 魏良兵[1] 宋俊梅[2] 

机构地区：[1]安徽中医药大学第一附属医院,国家中医药管理局中药制剂三级实验室,安徽合肥230031 [2]安徽中医药大学药学院,安徽合肥230038

出　　处：《中草药》2017年第12期2401-2406,共6页Chinese Traditional and Herbal Drugs

基　　金：国家中医药重点学科临床中药学建设项目(国中医药人[2012]32号教发);安徽中医药大学科学研究基金项目(2015fy003);2016年度省卫生计生委中医药科研课题(2016zy80)

摘　　要：目的建立补骨脂-肉豆蔻药对含药血清指纹图谱并分析补骨脂-肉豆蔻药对的入血成分。方法对20只SD大鼠分别ig 10个不同批次的补骨脂-肉豆蔻药对提取物,采用UPLC法比较体外供试品、含药血清和空白血清指纹图谱,分析其入血成分。结果测定给药后入血成分并建立UPLC指纹图谱,标出13个共有峰(相似度均在0.90以上),其中10个峰来源于体外供试品中原型成分,3个峰为代谢产物。结论首次采用血清药物化学方法,建立了补骨脂-肉豆蔻药对血清指纹图谱,反映补骨脂-肉豆蔻药对口服给药吸收入血情况,为其体内药效物质研究奠定基础。Objective To establish the chromatographic fingerprint of components of drug-couple Psoralea corylifolia-Myristica fragrants （PC-MF） absorbed into blood by UPLC. Methods Twenty SD rats were administered with 10 different batches of PC-MF respectively. The UPLC profiles of serum samples were collected after treatment of PC-MF, blank serum samples and methanol extract were compared, and the transitional constituents to blood and their metabolites were analyzed. Results The fingerprint of PC-MF was established. Thirteen common peaks were identified and the similarities were over 0.90. The 10 peaks from the in vitro test were prototype components in the product, and three peaks were metabolites. Conclusion The serum fingerprint of PC-MF is established for the first time. It reflects the oral administration absorption into the blood and provides some data on pharamacodynamic basis study in vivo for PC-MF.

关 键 词：补骨脂 肉豆蔻 药对 血清指纹图谱 超高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]