柴胡皂苷D通过逆转EMT抑制人成骨肉瘤细胞MG-63的迁移和侵袭  

Saikosaponin-D inhibits migration and invasion of osteosarcoma cell line MG-63 by reversing EMT

作　　者：张平安[1] 王晓伟[1] 郭向好 

机构地区：[1]陕西中医药大学第二附属医院骨科,陕西咸阳712000

出　　处：《现代肿瘤医学》2017年第16期2561-2564,共4页Journal of Modern Oncology

摘　　要：目的:研究柴胡皂苷D对人成骨肉瘤细胞MG-63的迁移和侵袭能力的影响并初步探讨其潜在分子机制。方法:运用MTT试验检测柴胡皂苷D对细胞增殖的影响,划痕试验和Transwell迁移试验检测柴胡皂苷D细胞迁移能力的影响,Transwell侵袭试验研究柴胡皂苷D对细胞侵袭能力的影响,Western-blot检测柴胡皂苷D对EMT相关蛋白的影响。结果:对MG-63增殖无明显影响浓度的柴胡皂苷D显著抑制细胞的迁移和侵袭能力(P<0.001),柴胡皂苷D上调E-cadherin的表达而下调N-cadherin和Vimentin的表达。结论:柴胡皂苷D通过逆转EMT改变抑制人成骨肉瘤细胞MG-63的迁移和侵袭。Objective：To study the impact of saikosaponin-D on human osteosarcoma cell line MG-63 migration and invasion,to explore the underlying mechanism.Methods：Using MTT assay to study the influence of saikosaponin-D on cell proliferation,wound healing assay and Transwell migration assay to study the influence of saikosaponin-D on cell migration,Transwell invasion assay to study the influence of saikosaponin-D on cell invasion.Western-blot was utilized to check the expression of EMT related proteins.Results：Saikosaponin-D which had no impact on cell proliferation significantly inhibited cell migration and invasion（P〈0.001）.Saikosaponin-D up-regulated the expression of E-cadherin and down-regulated the expression of N-cadherin and Vimentin.Conclusion：Saikosaponin-D inhibits migration and invasion of osteosarcoma cell line MG-63 by reversing EMT.

关 键 词：柴胡皂苷D 人成骨肉瘤 迁移和侵袭 EMT 

分 类 号：R730.53[医药卫生—肿瘤]