儿童急性髓系白血病免疫表型特点分析  

Analysis of lmmunophenotypic features of childhood acute myeloid leukemia

作　　者：王孝会[1] 陈芳[1] 符爽 胡延平[1] 张旻昱[1] 刘璇[1] 傅煜[1] 全铋 王绍坤[1] 张继红[1] 

机构地区：[1]中国医科大学附属盛京医院血液研究室,辽宁沈阳110022

出　　处：《现代肿瘤医学》2017年第16期2651-2654,共4页Journal of Modern Oncology

基　　金：国家自然科学基金青年基金(编号:81300420)

摘　　要：目的:分析儿童急性髓系白血病(AML)免疫表型特征,探讨其临床意义。方法:采用四色流式细胞术CD45/SSC双参数散点图设门方法,对127例儿童AML患者幼稚细胞进行免疫表型检测,对抗原表达情况进行分析。结果:在127例儿童急性髓系白血病患者中,髓系特异性抗原CD33、CD13和CD117的表达最常见,分别达95.3%、90.6%、90.6%。造血干/祖细胞抗原CD34、HLA-DR、CD38阳性率分别达53.5%、71.6%和97.6%。有65.4%的病例伴有淋系抗原的表达,其中以CD56的表达最常见占38.6%,其次为CD7占21.3%。有淋系抗原表达(LymAg^+)患者早期抗原CD34和HLA-DR抗原表达阳性率明显高于无淋系抗原表达(LymAg^-)患者(P<0.05)。结论:免疫分型对儿童AML的诊断和不同亚型鉴别具有重要意义。Objective：To investigate the immune phenotypic characteristics of childhood acute myeloid leukemia（AML） and its clinical significance.Methods：The immune phenotype in 127 cases with childhood AML were detected by four color flow cytometry with CD45/SSC double parameters scatter plot setting as door,and then the antigentic expressions were analysed.Results：In 127 patients with childhood AML,the Myeloid antigen of CD33,CD13,CD117 showed highly expressed.The positive rates were 95.3%,90.6%,90.6%.Hematopoietic stem/progenitor cell marker antigen CD34,HLA-DR and CD38 were expressed in AML with the rates of 53.5%,71.6% and 97.6%.The lymphoid antigen was positive in 65.4%of cases,with the highest frequency of CD56（38.6%）followed by CD7（21.3%）.The expression of CD34＋ and HLA-DR＋ patients in LymAg＋ group were significantly higher than LymAg-group（P〈0.05）.Conclusion：The immunophenotyping played an important role in diagnosis and classification of childhood AML.

关 键 词：急性髓系白血病 儿童 免疫表型 流式细胞术 

分 类 号：R733.7[医药卫生—肿瘤]