LPA_2对PC12细胞缺血再灌注损伤致细胞凋亡的作用  

The effect of LPA_2 on PC12 cell apoptosis induced by ischemia/reperfusion injury

作　　者：谷艳霞[1] 王超[1] 陈钜涛[1] 李婷婷[1] 王俐婷[1] 张杰[1] 曹来伟 张兆辉[1] 万芪[2] 

机构地区：[1]武汉大学人民医院神经内科,在读硕士研究生430060 [2]武汉大学基础医学院生理系

出　　处：《卒中与神经疾病》2017年第3期177-180,184,共5页Stroke and Nervous Diseases

基　　金：武汉大学医学部创新种子基金项目

摘　　要：目的探讨LPA_2在PC12细胞缺血再灌注损伤致细胞凋亡中发挥的作用。方法测最适缺氧时间:将PC12细胞分为6组,分别在三气培养箱中糖氧剥夺0、3、6、9、12、15 h。换高糖培养基普通培养箱中复氧24 h,MTT测细胞存活率;将PC12细胞分为5组:正常组、缺血再灌注组(OGD组)、缺血再灌注+溶剂对照组(OGD+DMSO组)、缺血再灌注+LPA_2激动剂(Dodecylphosphate)组(OGD+激动剂组)、缺血再灌注+LPA_2抑制剂(H2L5186303)组(OGD+抑制剂组),缺氧最适时间后再复氧24 h,MTT测细胞存活率,Western Blotting检测LPA_2及p-akt蛋白表达水平。结果缺氧处理12 h是模拟缺血再灌注损伤的最适时间;缺血再灌注组与正常组比较LPA_2表达水平降低;激动剂组与溶剂对照组比较LPA2表达水平增高,p-akt表达水平增高。结论升高LPA_2表达水平可提高细胞的存活率,LPA_2在缺血再灌注损伤中发挥保护作用。Objective To investigate the effect of LPA2 on PC12 cell apoptosis induced by ischemia/reperfusion injury.Methods To detect the suitable time of the oxygen glucose deprivation,the PC12 cells were divided into 6 groups, which were deprived of oxygen for 0,3, 6, 9, 12 and 15 h in tri-gas incubators. The cell viability was measured by MTT after 24 h of reoxygenation in a normal incubator.Then,the PC12 cells were divided into 5 groups：the normal culture group; ischemia/reperfusion group, ischemia/reperfusion group＋ solvent control（DMSO） group, Ischemia/reperfusion＋LPA2 antagonist（H2L5186303） group, ischemia/reperfusion＋LPA2 agonist （Dodecylphosphate） group. The cell viability was measured by MTT assay and the protein expression of LPA2 and p-akt were detected by western blotting.Results The oxygen glucose deprivation for 12 hours migat be the suitable time for inducing neuron injury. The expression of LPA2 receptor in OGD groups were lower than that in normal group and the expression of p-akt in the OGD group was lower than that in the normal control group. The expression of LPA2 receptor and p-akt in the agonist group were higher than that in the solvent control group.Conclusion Increasing the expression of LPA2 could improve the survival rate of cells, LPA2 played a protective role in PC12 cell apoptosis induced by ischemia/reperfusion injury.

关 键 词：缺血再灌注 糖氧剥夺 溶血磷脂酸受体2 PC12细胞 

分 类 号：R743.32[医药卫生—神经病学与精神病学]