Science:蛋白折叠远比想象中的更加复杂  

Hidden dynamics in the unfolding of individual bacteriorhodopsin proteins

作　　者：Hao Yu  Aric W. Sanders  Thomas T. Perkins 

机构地区：[1]JILA, National Institute of Standards and Technology and University of Colorado, Boulder, CO 80309, USA.[2]Department of Physics, University of Colorado, Boulder, CO 80309, USA.[3]Radio Frequency Technology Division, National Institute of Standards and Technology, Boulder, CO 80305, USA.[4]Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.

出　　处：《现代生物医学进展》2017年第18期I0001-I0001,共1页Progress in Modern Biomedicine

摘　　要：在一项新的研究中，来自美国国家标准技术研究所和科罗拉多大学博尔德分校的研究人员比以前更加详细地测量蛋白折叠，从而揭示出这种折叠过程比之前所知的更加复杂。这些结果提示着在此之前。对科学界而言，蛋白折叠在很大程度上仍然是未知的，这是因为这种折叠过程在如此短的时间内发生而且蛋白结构发生如此小的变化以至于常规的方法不能够检测出来。相关研究结果发表在Science期刊上。Elucidating the details of how complex proteins fold is a longstanding challenge. Key insights into the unfolding pathways of diverse proteins have come from single-molecule force spectroscopy (SMFS) experiments in which proteins are literally pulled apart. Yu et al. developed a SMFS technique that could unfold individual bacteriorhodopsin molecules in a native lipid bilayer with 1-µs temporal resolution (see the Perspective by Müller and Gaub). The technique delivered a 100-fold improvement over earlier studies of bacteriorhodopsin and revealed many intermediates not seen before. The authors also observed unfolding and refolding transitions between intermediate states.

关 键 词：蛋白折叠 科罗拉多大学 折叠过程 研究人员 标准技术 蛋白结构 研究所 

分 类 号：Q510.1[生物学—生物化学]