塞来昔布对大鼠氧诱导视网膜病变模型中新生血管的形态学影响  

Effects of celecoxib on morphological change of neovascularization in rat model of oxygen induced retinopathy

作　　者：任兵[1] 李鹏[2] 罗英[1] 高晓唯[1] REN Bing LI Peng LUO Ying et al(Ophthalmic Center,the 474th Hospital of PLA, Urumqi 830013, China)

机构地区：[1]解放军474医院全军眼科中心,新疆乌鲁木齐830013 [2]石河子大学医学院

出　　处：《西北国防医学杂志》2017年第6期361-364,共4页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:通过对大鼠氧诱导视网膜病变(oxygen-induced retinopathy,OIR)模型玻璃体腔注射塞来昔布,探讨塞来昔布在OIR中对视网膜新生血管的形态学影响。方法:7d龄SD乳鼠48只随机分为3组:对照组、ROP溶媒对照组、ROP+20μg塞来昔布组。对照组在正常环境中饲养,其余两组乳鼠均建立OIR模型,且于出生第12天出氧箱后给予玻璃体内注射二甲基亚砜(DMSO)及相应剂量的塞来昔布,于出生第17天处死,做组织切片HE染色,观察视网膜组织形态并计数突破内界膜的血管内皮细胞核数。结果:HE染色显示,3组突破视网膜内界膜的血管内皮细胞核数分别为(0.44±0.18)、(30.60±5.36)、(10.13±1.93)个,各组间差异均有统计学意义(均P<0.05)。经塞来昔布治疗后,突破内界膜的血管内皮细胞核明显减少。结论:塞来昔布能够有效抑制大鼠OIR模型视网膜新生血管的生长。Objective： To investigate the effects of celecoxib on morphological change of neovascularization in oxygen induced retinopathy （OIR）, through intravitreal injection of celecoxib in rat model of OIR .Methods：Forty-eight 7 day old SD rats were randomly divided into 3 groups ： control group, ROP group, ROP＋ 20 μg celecoxib group.Control group received normal partial oxygen pressur,ROP group, ROP＋ 20 μg celecoxib group were established the OIR model.The neonatal rats were given intravitreal injections of DMSO and the corresponding doses of celecoxib on the 12th day after birth.The rats were sacrificed on the 17th after birth, HE staining were employed to count the vascular endothelial cells which were breakthrough within the internal limiting membrane of retina.Results： HE staining showed that the number of the endothelial cells in the retina was （0.44 ±0.18）, （30.60 ±5.36 ）, （10.13 ± 1.93） in three groups,there were significant differences between the three groups （all P 〈0.05）, after treatment with celecoxib,breakthrough of the internal limiting membrane of the vascular endothelial cell nucleus was significantly reduced, and positively correlated with the dose. Conclusion. Celecoxib can inhibit the OIR model of rat retinalangiogenesis.

关 键 词：视网膜病变 塞来昔布 新生血管 

分 类 号：R-332[医药卫生] R774.1