幽门螺杆菌联合MNU灌胃法制备balb/c小鼠胃癌模型研究  

Helicobacter pylori combined with MNU gavage for preparing balb/c mouse gastric cancer model

作　　者：孙艳珍[1] 张涛[1] 陈良荣[1] 欧超[2] 黄晓燕[3] 杨杰[1] 陈远能[1] 

机构地区：[1]广西中医药大学附属瑞康医院消化内科,南宁530011 [2]广西壮族自治区肿瘤防治研究所检验科,南宁530021 [3]广西中医药大学第一附属医院消化内科,南宁530023

出　　处：《重庆医学》2017年第20期2806-2808,2811,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81360531);广西卫生厅重点项目(重2012030)

摘　　要：目的探讨幽门螺杆菌联合N-甲基-N-亚硝基脲(MNU)灌胃法制备balb/c小鼠胃癌模型。方法 80只balb/c小鼠,适应性喂养1周后,分成4组,即正常组、模型Ⅰ组、模型Ⅱ组、模型Ⅲ组,每组20只。模型Ⅰ组、模型Ⅱ组、模型Ⅲ组,先予幽门螺杆菌菌液CFU=109/mL灌胃,隔天1次,共5次;然后依次分别给予新鲜配制的MNU溶液0.15、0.30、0.60mL灌胃,MNU和纯水配置比为5mg∶3mL,每周灌胃1次,持续10周。结果模型Ⅱ组66.67%腺癌,模型Ⅰ组小鼠全部胃炎伴轻度不典型增生,模型Ⅲ组小鼠全部死亡。结论此法能够制备胃癌模型。Objective To investigate Helicobacter pylori combined with N-methyl-N-nitrosourea （MNU） gavage for prepar- ing balb/c mouse gastric cancer model. Methods Eighty balb/c mice were randomly divided into four groups after 1-week adaptive feed,normal group,model group Ⅰ , Ⅱ and Ⅲ,20 cases in each group. The model group Ⅰ , Ⅱand Ⅲ were given Helicobacter py- lori bacteria liquid （CFU= 109/mL） gavage, once every other day for 5 times;then, the freshly configured MNU solution 0.15,0.3, 0.6 mL gavages were in turn given,MNU and pure water allocation ratio was 5mg：3mL. Once gavage per week for continuous 10 weeks. Results The model group Ⅱ had 66.67% adenocarcinoma,the model group Ⅰ were gastritis with mild atypical hyperplasia, and all mice in the model group III died. Conclusion This method can prepare the gastric cancer model.

关 键 词：N-甲基-N-亚硝基脲 螺杆菌 幽门 BALB/C小鼠 胃肿瘤 

分 类 号：R-332[医药卫生] R735.2