血清STAT1和肿瘤标志物联合检测对胃癌的诊断价值  

Diagnosis value of combined detection of STAT1 and tumor markers in gastric carcinoma

作　　者：袁建明[1] 刘涛[1] 沈东杰[1] 沈建康[1] 周儒[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院卢湾分院外科,上海200020

出　　处：《生物医学工程与临床》2017年第4期425-428,共4页Biomedical Engineering and Clinical Medicine

基　　金：上海市卫生和计划生育委员会基金资助项目(20134461)

摘　　要：目的检测胃癌患者血清信号传导及转录激活因子1(STAT1)和传统肿瘤标志物的表达,探讨血清STAT1与肿瘤标志物的联合检测对胃癌的诊断价值。方法选择83例胃癌患者(胃癌组),其中男性51例,女性32例;年龄32~74岁,平均年龄60.02岁。同期40例胃良性溃疡患者(溃疡组),其中男性24例,女性16例;年龄35~68岁,平均年龄58.85岁。40例健康志愿者(正常对照组),其中男性21例,女性19例;年龄35~65岁,平均年龄58.75岁。取其血清标本,应用酶联免疫吸附分析(ELISA)方法检测STAT1的表达,并检测肿瘤标志物[癌胚抗原(CEA)、糖类抗原(CA)19-9、CA72-4、CA50、甲胎蛋白(AFP)]的表达,比较不同分期胃癌患者STAT1和肿瘤标志物的表达。结果胃癌组血清STAT1平均浓度(66.38±48.72)μg/L,显著低于溃疡组[(120.98±52.26)μg/L](P=0.000)和正常对照组[(93.15±36.92)μg/L](P=0.006)。STAT1+5项肿瘤标志物联合检测可提高灵敏度(42.17%vs 34.94%)(P=0.028),特异度差异无统计学意义(77.50%vs 83.75%)(P=0.257)。血清STAT1在胃癌各期患者中的灵敏度差异无统计学意义(P=0.923)。结论血清STAT1的表达与胃癌的发病密切相关,可作为传统肿瘤标志物的补充,对胃癌的早期诊断有积极意义。Objective To detect the expressions of serum signal transducers and activators of transcription 1（STAT1） and tumor markers, and discuss the diagnosis value of combined detection in gastric carcinoma. Methods A total of 83 cases with gastric carcinoma（gastric carcinoma group） were enrolled, included 51 males and 32 females, aged 32-74 years old with mean age of 60.02 years old. Forty serum cases of benign gastric ulcer patients were enrolled as ulcer group, included 24 males and 16 females, aged 35-68 years old with mean age of 58.85 years old. Forty serum samples of healthy subjects as normal control group, included 21 males and 19 females, aged 35-65 years old with mean age of 58.75 years old. The expressions of serum STAT1 and tumor markers were detected, the expressions of STAT1 and tumor markers[carcino-embryonic antigen（CEA）, carbohydrate antigen 19-9（CA19-9）, carbohydrate antigen 72-4（CA72-4）, carbohydrate antigen 50（CA50） and alpha fetal protein（AFP）] in different stages of gastric carcinoma were compared. Results The mean level of serum STAT1 in gastric carcinoma group was（66.38 ± 48.72） μg/L, which was significantly lower than that of ulcer group[（120.98 ± 52.26） μg/L]（P = 0.000） and normal control group[（93.15 ± 36.92） μg/L]（P = 0.006）. Compared with tumor markers, the sensitivity was significantly improved in combined with STAT1（42.17 % vs 34.94 %）（P = 0.028）, but specificity was no significantly different（77.50 %vs 83.75 %）（P = 0.257）. No other significant differences were observed in different pathological staging of gastric carcinoma（P = 0.923）. Conclusion It is demonstrated that the expression of serum STAT1 is closely related to the development of gastric carcinoma, which can be used as supplement for tumor markers, and especially has a high value to the diagnosis of early gastric carcinoma.

关 键 词：血清信号传导及转录激活因子1(STAT1) 肿瘤标志物 胃癌 癌胚抗原(CEA) 糖类抗原19-9(CA19-9) 糖类抗原72-4(CA72-4) 糖类抗原50(CA50) 甲胎蛋白(AFP) 血清 

分 类 号：R735.2[医药卫生—肿瘤]