大剂量甲强龙在BMSC移植治疗大鼠脊髓损伤中的作用  

Effect of high-dose methylprednisolone on spinal cord injury in rats treated with BMSC transplantation

作　　者：成垚昱 杨新明[2] 张振梁[1] 康聪[1] 

机构地区：[1]河北北方学院,河北张家口075000 [2]河北北方学院附属第一医院

出　　处：《山东医药》2017年第21期13-16,共4页Shandong Medical Journal

基　　金：河北北方学院创新人才培育基金项目(20110176);河北省卫生厅医学科学研究重点项目计划(CXRC1322)

摘　　要：目的探讨大剂量甲强龙(MP)在BMSC移植治疗大鼠脊髓损伤中的作用。方法采用改良Allen法制备72只T_(10)脊髓损伤雄性大鼠模型,随机均分为对照组、MP组、BMSC组、MP+BMSC组。MP组造模后立即经尾静脉推注MP 30 mg/kg;BMSC组造模后2 h注射Brd U标记的BMSC悬液2 mL;MP+BMSC组给予以上两种干预方法。分别于造模后第1、7、14天采用运动评分标准(BBB)评价大鼠后肢运动功能,光学显微镜下观察脊髓组织形态学变化,用免疫组化法检测大鼠脊髓组织TNF-α、Caspase-3表达,用Brd U染色检测脊髓组织中移植BMSC存活率。结果造模后第7、14天,MP+BMSC组BBB评分与其他三组比较,P均<0.05;对照组组织及细胞水肿最显著,神经元存活最少,MP组、BMSC组次之,MP+BMSC组水肿最轻,神经元存活最多;MP+BMSC组TNF-α、Caspase-3阳性细胞数与其他三组比较,P均<0.05;MP+BMSC组移植BMSC存活率与BMSC组比较,P<0.05。结论 MP促进BMSC移植治疗脊髓损伤大鼠后肢运动功能的恢复,降低损伤局部TNF-α的表达;并且MP有效促进移植BMSC的存活。Objective To investigate the effect of high dose of methylprednisolone （MP） on spinal cord injury （SCI） in rats treated with bone marrow mesenchymal stem cell （BMSC） transplantation. Methods Seven-two male T10 SCI models were prepared by modified Allen method. The rats were randomly divided into the control group, MP group （which was injected with MP 30 mg/kg）, BMSC group （which was injected with 2 mL BMSC suspension after 2 h of model formation）, and MP＋BMSC group （which was treated with the above two intervention methods）. The motor function of the hind limbs was evaluated by BBB score on day 1, 7 and 14 after modeling, and morphological changes of spinal cord were observed under light microscope. The expression of TNF-α and Caspase-3 in the spinal cord of rats was detected by immunohistochemistry. The cell viability of transplantated BMSCs was detected by BrdU staining in spinal cord tissues. Results Compared with the other three groups, the BBB score of MP ＋ BMSC group was statistically significant on day 7 and 14 after modeling （P〈0.05）. Histological showed edema and neuronal viability were significant in the control group with the least survival neurons, followed by MP group and BMSC group, and the MP＋BMSC group was the slightest with the most survival neurons. There were significant differences in TNF-α and the number of Caspase-3 positive cells between MP＋BMSC group and the other three groups （all P〈0.05）. The neuronal viability of transplantated BMSCs in MP ＋ BMSC group was higher than that in BMSC group （P〈0.05）. Conclusion MP facilitates the hind limb motor function recovery in SCI rats treated with BMSC transplantation, reduces the level of local TNF-α expression, and effectively promotes the survival of transplanted BMSC.

关 键 词：脊髓损伤 甲强龙 骨髓间充质干细胞 细胞移植 肿瘤坏死因子Α 

分 类 号：R744[医药卫生—神经病学与精神病学]