广东汉族人群HBV基因型、基本核心启动子/前C区突变与肝癌易感相关性研究  

Hepatitis B virus genotype and BCP/Pre-C region gene polymorphisms in susceptibility to hepatocellular carcinoma among the Chinese Han population in Shenzhen,Guangdong

作　　者：刘树旺[1] 张丽芬[1] 刘利平[1] 刘林森[1] 张悦[1] 李琪[1] 彭公泽 鲍世韵[1] 

机构地区：[1]暨南大学第二临床医学院(深圳市人民医院)肝胆胰外科,518020

出　　处：《临床外科杂志》2017年第6期444-447,共4页Journal of Clinical Surgery

基　　金：深圳市科技创新资助项目(JCYJ20160422152408705;JCYJ20150403101028198)

摘　　要：目的研究广东汉族肝癌HBV基因型、基本核心启动子(BCP)/前C区突变与肝癌易感性的相关关系。方法按照病例对照研究设计方法,纳入HBV合并肝癌患者(病例组)100例和HBV携带者(对照组)100例,收集血清样本,提取HBV DNA,采用巢式PCR扩增HBV S区、BCP及前C区片段,双向测序,分析HBV基因型、BCP/前C区基因突变与HBV合并肝癌易感性的相关性。结果病例组BCP区A1762T/G1764A突变及前C区T1858C突变高于对照组,分别为92.0%和73.0%(P<0.05)、52.0%和32.0%(P<0.05);对照组A1775G突变发生率高于病例组,分别为31.0%和14.0%(P<0.05)。多因素Logistic回归分析显示,HBV BCP区A1762T/G1764A突变和前C区T1858C突变与HBV合并肝癌发生独立相关[OR 6.508(95%CI:1.287~16.739,P<0.05)和3.371(95%CI:2.268~8.163,P<0.05)];而BCP区A1775G突变则为保护因素[OR=0.163(95%CI:0.043~0.489,P<0.05)]。结论 HBV C基因型是广东深圳地区HBV合并肝癌的主要流行株,HBV BCP区A1762T、G1764A、A1775G、前C区T1858C基因多态性与HBV合并肝癌的易感性具有密切关系。Objective To investigate the relationship between hepatitis B virus（ HBV） genotype,polymorphisms in basic core promoter（ BCP） region/pre-core（ Pre-C） region and the incidence of hepatocellular carcinoma（ HCC） in Guangdong Province. Methods In this case-control study,100 HCC patients and 100 asymptomatic HBV carriers were included. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype,gene mutation of HBV and the incidence of HCC was analyzed. Results The mutation rates of the A1762T/G1764 A in the BCP region and the T1858 C in the Pre-C region of HBV were significantly higher in HCC group than in control group（ 92% vs. 73%,P〈0. 05; 52% vs. 32%,P〈0. 05）. The mutation rate of A1775 G was significantly higher in control group（ 31%） than in HCC group（ 14%）（ P〈0. 05）.Binary logistic regression analysis indicated that A1762T/G1764 A and T1858 C mutations are the risk factors for the development of HCC（ OR 6. 508,95% CI： 1. 287 ~ 16. 739,P〈0. 05 and 3. 371,95% CI：2. 268 ~ 8. 163,P〈0. 05）. A1775 G is a protective factor in the development of HCC（ OR = 0. 163,95%CI： 0. 043 ~ 0. 489,P〈0. 05）. Conclusion BCP A1762T/G1764 A,A1775G and Pre-C T1858 C mutations are correlated with the incidence of HCC in Guangdong.

关 键 词：肝癌 乙型肝炎病毒 HBV基因型 突变 

分 类 号：R512.62[医药卫生—内科学]