DBP高效降解菌群的富集及其降解特性研究  

Enrichment of a highly efficient DBP-degrading bacterium consortium and its degradation characters

作　　者：李方方[1] 宋建[1] 吕律[1] 王洋洋[1,2] 翟秋敏[1] 杨志辉[3] 柴立元[3] 

机构地区：[1]河南大学资源与环境研究所,开封475004 [2]黄河文明与可持续发展研究中心,开封475004 [3]中南大学冶金与环境学院,长沙410083

出　　处：《环境科学学报》2017年第7期2579-2585,共7页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.41430637);河南省高校科技创新团队支持计划资助(No.16IRTSTHN012);河南省教育厅科学技术研究重点项目(No.16A610015);河南省科技发展计划项目(No.152102310297)~~

摘　　要：以邻苯二甲酸二丁酯(DBP)为唯一碳源及能源,从开封市垃圾焚烧发电厂活性污泥中富集得到细菌菌群LV-1,高通量测序显示LV-1共包含33个科、48个属,其中优势菌属为布鲁氏杆菌(Brucella sp.,62.78%)和中华杆菌(Sinobacter sp.,14.83%).在最佳降解条件下(30℃、p H6.0),LV-1 48 h内可将500 mg·L-1DBP降解93%左右,72 h内将1000 mg·L-1DBP降解95%以上.当DBP浓度为100~500 mg·L^(-1)时,LV-1对DBP的降解过程符合一级动力学方程,半衰期为13.64~19.20 h.利用GC-MS对DBP生物降解的中间产物进行分析,推测LV-1降解DBP的生化途径:DBP—→邻苯二甲酸-1-乙基-6-丁基酯(BEP)—→邻苯二甲酸单丁酯(MBP)—→邻苯二甲酸单乙酯(MEP)—→邻苯二甲酸(PA—→—→)CO_2+H_2O.此外,LV-1还能够有效降解侧链较短的邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)及PA.A bacteria consortium（ LV-1）,which can utilize di-n-butyl phthalate（ DBP） as sole source of carbon and energy,was isolated from activated sludge collected from a garbage incineration power plant of Kaifeng. Based on the results of Illumina sequencing,LV-1 contains 33 families and 48 genera of bacteria,the dominant genera is Brucella sp. and Sinobacter sp. and their relative abundance is 62. 78% and 14. 83%,respectively. The optimal degradation condition of LV-1 to degrade DBP was： temperature 30 degrees centigrade and p H 6.0. Under the optimum conditions,LV-1 degraded about93% of 500 mg·L-1DBP within 48 h,and more than 95% of 1000 mg·L-1DBP was degraded within 72 h. The investigation of degradation kinetics at different DBP initial concentrations（ 100 ~ 500 mg·L-1） showed that DBP degradation fitted the Monod first-order kinetic equation,and the half-life ranged from 13. 64 to 19. 20 h. Gas chromatography-mass spectrometry（ GC-MS） was used to analysis the intermediates during the DBP degradation process,and the degradation pathway was proposed： DBP—→BEP—→MBP—→MEP—→PA—→—→CO_2＋ H_2O.Besides,LV-1 can effectively utilize DMP,DEP and PA.

关 键 词：邻苯二甲酸二丁酯 细菌菌群 生物降解 降解途径 

分 类 号：X172[环境科学与工程—环境科学]