富半胱氨酸蛋白61在肝卵圆细胞增殖和分化中的作用及意义  

Effect of cyr61 on proliferation and differentiation of hepatic progenitor cells

作　　者：丁伟 谭洪波 李雪梅 姜昌丽 蔡第心 张悦 潘兴华 

机构地区：[1]昆明总医院检验科,昆明市650032 [2]昆明总医院骨科,昆明市650032 [3]昆明总医院细胞生物治疗中心,昆明市650032

出　　处：《实用医学杂志》2017年第13期2109-2112,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81360274;31200657);昆明市2016年西山区科技计划项目(编号:西科字19号)

摘　　要：目的分析肝卵圆细胞(HOCs)在2-乙酰氨基芴+2/3肝切除(2-AAF/PHx)大鼠肝损伤模型中的增殖、分化情况,并且对富半胱氨酸蛋白61(Cyr61)在HOCs增殖、分化中的作用及机制进行初步研究。方法构建2-AAF/PHx大鼠肝损伤模型,采用免疫组织化学染色、Western blot检测等方法对大鼠肝脏组织中HOCs增殖、分化情况和Cyr61的表达水平进行检测分析;并且,采用Western blot检测等方法研究分析HOCs株WB-F344被诱导分化的过程中,Cyr61和β-连环蛋白(β-catenin)表达水平的变化。结果2-AAF/PHx大鼠模型肝脏组织中Cyr61的表达在HOCs活化增殖的过程中明显升高,HOCs分化为正常肝细胞或胆管细胞后又恢复正常水平;体外实验中,Cyr61和β-catenin蛋白表达水平在WB-F344细胞向肝细胞分化过程中明显增高。结论 Cyr61可以促进HOCs的增殖和分化,并且可能和β-catenin信号通路的激活密切相关。Objective To investigate the regeneration and differentiation of HOCs in the 2-AAF/PHx rat models. To explore the expression of Cyr61and its mechanism in differentiation of HOCs in vitro. Methods In 2- AAF/PHx rats model, induction and expansion of HOCs were detected by immunochemistry and HE staining. Western blot was used for observing the expression of Cyr61. Furthermore, the expression of Cyr61 and [3-catenin were detected by Western blot in differentiation of WB-F344 cells in vitro. Results Cyt61 protein level increased as a result of HOCs in rats livers after 2-AAF/PHx. In addition, the expression of Cyr61 and β-catenin significantly increased during WB-F344 cells differentiation in vitro. Conclusions Cyr61 might play an important role as a signaling mediator in HOCs response and closely correlate with Cyr61 and β-catenin in proliferation and differentiation of HOCs.

关 键 词：富半胱氨酸蛋白61 Β-连环蛋白 肝卵圆细胞 2-乙酰氨基芴 

分 类 号：R735.7[医药卫生—肿瘤]