沉默FOXM1拮抗肝细胞生长因子对胃癌细胞SGC7901增殖、迁移和侵袭的诱导作用  

Silencing of FOXM1 gene inhibits HGF-induced proliferation,migration and invasion of gastric cancer SGC7901 cells

作　　者：王海芳[1] 赵向绒[1] 霍雪萍[1] 武祥龙[2] 牛银波[2] 

机构地区：[1]陕西省人民医院中心实验室,陕西西安710068 [2]西北工业大学生命学院,陕西西安710072

出　　处：《现代肿瘤医学》2017年第15期2383-2387,共5页Journal of Modern Oncology

摘　　要：目的:探索转录因子FOXM1在肝细胞生长因子(HGF)诱导胃癌细胞增殖、迁移和侵袭过程中的作用。方法:体外培养胃癌细胞SGC7901,根据不同处理将细胞分组:空白对照组、20 ng/ml HGF组、40 ng/ml HGF组、60 ng/ml HGF组、siRNA-scramble+HGF组、siRNA-FOXM1+HGF组。采用MTT法检测各组SGC7901细胞的增殖,Transwell法检测各组细胞的迁移和侵袭情况,qRT-PCR和Western blot法分别检测各组细胞中FOXM1 mRNA和蛋白水平。结果:与空白对照组相比,不同浓度HGF均能够促进胃癌细胞SGC7901增殖、迁移和侵袭,同时上调FOXM1的表达水平,并且呈浓度依赖性关系。沉默HOXM1表达能够明显抑制HGF对SGC7901细胞的增殖、迁移和侵袭的诱导作用。结论:HGF对胃癌细胞增殖、迁移和侵袭的诱导作用可能与上调FOXM1表达有关,沉默FOXM1表达能够抑制HGF的作用。Objective： To study the role of FOXM1 in proliferation,migration and invasion of gastric cancer SGC7901 cells induced by hepatocyte growth factor（ HGF）. Methods： SGC7901 cells were cultured and divided into groups with different treatments. Control group,20 ng/ml HGF group,40 ng/ml HGF group,60 ng/ml HGF group,siRNA-scramble ＋ HGF group and siRNA-FOXM1 ＋ HGF group. Cell proliferation was detected by MTT assay,Transwell assay was performed to evaluate cells migration and invasion,while the mRNA and protein level of FOXM1 was measured by qRT-PCR and Western blot,respectively. Results： Compared with the control group,different concentrations of HGF promoted SGC7901 cells proliferation,migration and invasion,meanwhile upregulated the expression of FOXM1 in a concentration-dependent manner. Silencing of FOXM1 gene significantly inhibited HGF-induced SGC7901 cells proliferation,migration and invasion. Conclusion： The induction of HGF on SGC7901 cells proliferation,migration and invasion may be related with the up-regulation of FOXM1 expression,silencing of which significantly inhibited the effect of HGF.

关 键 词：FOXM1 肝细胞生长因子 胃癌 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]