血小板膜糖蛋白482C/T单核苷酸多态性与肺癌的相关性  

Relevance between Platelet Membrane Glycoprotein 482C/T Single Nucleotide Polymorphisms and Lung Carcinoma

作　　者：杨芳[1,2] 王伟[2] 彭云潇 邵春燕[2] 符贵 YANG Fang WANG Wei PENG Yunxiao SHAO Chunyan FU Gui(Guizhou Medical University, Guiyang 550003, Guizhou, China Guizhou Provincial Tumor Hospital, Guiyang 550004, Guizhou, China)

机构地区：[1]贵州医科大学附属肿瘤医院,贵州贵阳550003 [2]贵州省肿瘤医院,贵州贵阳550004

出　　处：《贵州医科大学学报》2017年第7期794-798,共5页Journal of Guizhou Medical University

基　　金：贵州省科技厅联合基金项目(TN2014-73)

摘　　要：目的:探讨血小板膜糖蛋白(GP)482C/T位点单核苷酸多态性与肺癌发生及发展的关系。方法:收集100例肺癌患者和100例健康体检者的外周血,采用序列特异性引物-聚合酶链反应技术方法检测482C/T位点单核苷酸多态性基因型;应用Hardy-Weinberg(H-W)平衡软件计算肺癌患者基因型的分布并进行统计检验,采用χ~2检验分析肺癌患者基因型和等位基因频率的分布,非条件logistic回归法计算比值比(OR)及95%CI;对GP-482C/T基因不同基因型与肺癌的相关性进行相对危险度分析。结果:肺癌组GP-482C/T基因型频率及等位基因频率与对照组比较,差异有统计学意义(P<0.05),淋巴结转移组GP-482C/T等位基因频率与淋巴结未转移组比较,差异有统计学意义(P<0.05);GP-482C/T位点基因型频率及其等位基因频率在肺癌各临床病理分期中比较差异无统计学意义(P>0.05);GP-482C/T位点的不同基因型及其等位基因频率与肺癌的的相关性进行相对危险度分析,结果显示GP-482C/T位点中CT型及T等位基因对肺癌的发病有统计学意义(OR值=0.112,0.211;95%CI=0.059~0.215,0.122~0.364;P<0.05)。结论:GP-482C/T位点CC基因型可能与肺癌的发病存在相关性。Objective： To investigate the relationship between the occurrence and development between platelet membrane glycoprotein 482C/T polymorphisms and lung carcinoma. Methods： Collecting peripheral blood of 100 patients with lung carcinoma and 100 healthy people, adopting sequence specific primers polymerase chain reaction technique to detect 482C/T site single nucleotide polymor- phisms genotypes. Adopting Hardy-Weinberg （H-W） balancing software to calculate distribution of ge- no-type of lung carcinoma patients for statistics test; adoptingχ2 test to analyze geno-type and distribu- tion of allele frequency; adopting unconditioned logistic regression method to calculate odds ratio （OR） and 95% CI; analyzing relative risk levels on relevance between various geno-types and lung carcinoma of GP-482C/T genes. Results： The frequencies of GP-482C/T genotype and allele frequen- cy of lung carcinoma group were statistically significant compared with the control group （ P 〈 0.05 ）. Comparing allele frequency of GP-482C/T of lymphoglandula metastasis group with non-metastasis group, differences were statistically significant （ P 〈 0.05 ） ; comparison of lung carcinoma group GP- 482C/T genotype polymorphism genotype frequency and allele frequency had no significant difference in lung carcinoma pathological stage （ P 〉 0.05 ） ; relative risk level analysis relevance between various geno-type of GP-482C/T site point and lung carcinoma showed CT type and T allele of GP482C/T site point had statistical significance on pathogenesis of lung carcinoma（ OR =0. 112,0. 211; 95% CI = 0. 059-0. 215, 0. 122 -0. 364; P 〈0.05）. Conclusion： GP-482C/T site may be related to the pathogenesis of lung carcinoma.

关 键 词：肺肿瘤 血小板膜糖蛋白类 多态性 单核苷酸 关联性 

分 类 号：R734.2[医药卫生—肿瘤]