蓝光对人视网膜色素上皮细胞增殖的影响和机制研究  

Effect of blue light on proliferation of human retinal pigment epithelial cells

作　　者：朱红娜[1] 乔瑛[1] 苏安乐 张婷[1] 孙中洋[2] 梁厚成[1] Hong- Na Zhu Ying Qiao An - Le Su Ting Zhang Zhong-Yang Sun Hou-Cheng Liang(Department of Ophthalmology, Xi＇an NO. 1 Hospital, Xi＇an 710002, Shaanxi Province, China the 454 Hospital of PLA, Nanjing 221002, Jiangsu Province, China)

机构地区：[1]中国陕西省西安市第一医院眼科,710002 [2]解放军第四五四医院,中国江苏省南京市210002

出　　处：《国际眼科杂志》2017年第8期1419-1422,共4页International Eye Science

基　　金：国家自然科学基金青年基金项目(No.81600694)~~

摘　　要：目的:研究蓝光对人视网膜色素上皮细胞增殖能力的影响,并初步探讨其可能机制。方法:利用35W白色冷光灯加用蓝色滤光片建立蓝光损伤体外培养的RPE细胞模型,蓝光控制波长在470~520nm,光照强度控制为2000Lx左右,光照时间控制为24~96h,利用CCK-8法检测RPE细胞的增殖能力,利用real-time PCR技术检测RPE细胞中miR-103的含量。结果:与对照组相比,蓝光照射组的RPE细胞的增殖能力减弱;蓝光照射组的RPE细胞内的miR-103含量较对照组增加;miR-103过表达时,RPE细胞的增殖能力减退,降低miR-103的表达时,细胞增殖能力增强;降低miR-103的表达能够减弱蓝光对RPE细胞增殖的抑制作用。结论:蓝光通过上调miR-103抑制RPE细胞的增殖,miR-103可能成为治疗年龄相关性黄斑变性等视网膜变性疾病治疗的新靶点。AIM： To investigate the influence and mechanism of blue light on the proliferation of human retinal pigment epithelial cells.METHODS： Cells were divided into two groups,including blue light group and control group.The 35W white light lamp with blue filter was used to establish damaged RPE cell model in vitro.Blue ray wavelength ranged between 470nm and 520nm.And the light intensity was about 2000Lx.After exposure to blue light,we tested the proliferation of human retinal pigment epithelial cells by CCK-8 kit.And then expression of miR-103 was measured by the real-time PCR.RESULTS： Exposure to blue light inhibited the proliferation of human retinal pigment epithelial cells and increased the expression of miR-103.Moreover,up-regulation of miR-103 inhibited the proliferation of human retinal pigment epithelial cells,and down-regulates miR-103 promoted the proliferation of human retinal pigment epithelial cells.CONCLUSION： Blue light inhibits the proliferation of human retinal pigment epithelial cells by the up-regulation of miR-103.

关 键 词：蓝光 人视网膜色素上皮细胞 增殖 miR-103 

分 类 号：R774[医药卫生—眼科]