五指山小型猪、西藏小型猪和巴马小型猪对外源性脂肪的敏感性观察  

Observation on the sensitivity of Wuzhishan,Tibetan and Bama minipigs to exogenous fats

作　　者：陈亮[1] 潘永明[1] 朱科燕[1] 徐孝平[1] 徐剑钦[1] 陈民利[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中国比较医学杂志》2017年第6期12-16,共5页Chinese Journal of Comparative Medicine

基　　金：浙江省科技厅实验动物科技计划项目(2012C37078);浙江中医药大学比较医学创新团队(XTD201301)

摘　　要：目的观察五指山小型猪、西藏小型猪和巴马小型猪对外源性脂肪的敏感性。方法取雄性五指山小型猪、西藏小型猪和巴马小型猪各5只,分别采用静脉注射脂肪乳和饲喂高脂饲料,分别检测静脉注射脂肪乳后0.5、1、1.5、2、2.5、3 h和饲喂高脂饲料后12、24、36、48、60、72、84、120 h的血清胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(LDL-C)和甘油三酯(TG)水平,并计算曲线下面积变化ΔAUC值。结果注射脂肪乳后3种小型猪甘油三酯(TG)均明显升高(P<0.01),在注射后0.5 h达到峰值,其升高程度和ΔAUC值高低依次为西藏小型猪>五指山小型猪>巴马小型猪,而TC、LDL-C和HDL-C则无明显变化(P>0.05);高脂饲料饲喂饮食诱导后3种小型猪TC、LDL-C、HDL-C和TG均明显升高(P<0.05,P<0.01),其中,五指山小型猪TC、LDL-C在饲喂后36 h、HDL-C在饲喂后48 h、TG在饲喂后24 h达到峰值,巴马小型猪和西藏小型猪TC、LDL-C、HDL-C在饲喂后48-60 h、TG在饲喂后36 h达到峰值,TC、LDL-C、HDL-C的ΔAUC值高低依次为五指山小型猪>巴马小型猪>西藏小型猪。结论静脉注射脂肪乳3种小型猪对TG敏感,脂耐量值高低依次为西藏小型猪>五指山小型猪>巴马小型猪;高脂饲料饲喂对3种小型猪TC、LDL-C、HDL-C敏感,脂耐量值高低依次为五指山小型猪>巴马小型猪>西藏小型猪。Objective To observe the sensitivity of Wuzhishan,Tibetan and Bama minipigs to exogenous fats.Methods A total of 15 male minipigs including 5 WZS minipigs,5 Tibetan minipigs and 5 Bama minipigs,were used in this study. The minipigs were intravenously injected with fat emulsion and fed with high-fat diet,and the changes of serum total cholesterol（ TC）,low density lipoprotein（ LDL-C）,high density lipoprotein（ HDL-C） and triglyceride（ TG） levels were detected at 0. 5 h,1 h,1. 5 h,2 h,2. 5 h and 3 h after intravenous injection of fat emulsion and at 3 h and 12 h,24 h,36 h,48 h,60 h,72 h and 84 h after fed with high-fat diet,respectively,and calculated the changes of area under the curve（ ΔAUC） values. Results The triglyceride（ TG） in the three kinds minipigs were significantly increased（ P 〈0. 01）,and reached a peak at 0. 5 h after injection. The degree of elevation of ΔAUC values were different showing on increasing order： Tibetan minipig Wuzhishan minipig Bama minipig,while TC,LDL-C and HDL-C showed no significant changes（ P 〈0. 05）. Moreover,TC,LDL-C,HDL-C and TG were significantly increased in the three stocks of minipigs induced by feeding with high-fat diet（ P 〈0. 05,P 〈0. 01）. Among them,the TC and LDL-C of Wuzhishan minipigs peaked at 36 h,HDL-C peaked at 48 h and TG peaked at 24 h after feeding,respectively. TC,LDL-C and HDLC in the Bama minipigs and Tibetan minipigs peaked at 48-60 h and TG peaked at 36 h after feeding,and the ΔAUC values of TC,LDL-C and HDL-C were in an increasing order of Wuzhishan minipigs Bama minipigs Tibetan minipigs. Conclusions The three stocks of minipigs are sensitive to TG after intravenous injection of fat emulsion,and the lipid tolerance values are in an order of Tibetan minipig Wuzhishan minipigs Bama minipigs. Meanwhile,the three stocks of minipigs are also sensitive to TC,LDL-C and HDL-C after feeding with high-fat diet,and the lipid tolerance values are in an increasing order of Wuzhishan minipig Bama minipigs Tibetan minipig

关 键 词：小型猪 血脂代谢 脂耐量 敏感性 

分 类 号：R-33[医药卫生]