PD-1/PD-L1在膀胱癌患者外周血T细胞和肿瘤细胞表达  

PD-1/PD-L1 Expressed on T Cell and Bladder Cancer Cell

作　　者：黄韬[1] 龙兆麟[1] 吴世皓[1] 华庆生[1] 张信基[1] 

机构地区：[1]南方医科大学附属顺德第一人民医院泌尿外科,广东顺德528300

出　　处：《中山大学学报（医学科学版）》2017年第4期628-631,F0003,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81272844)

摘　　要：【目的】探讨PD-1/PD-L1在膀胱肿瘤患者外周血T细胞和膀胱肿瘤细胞上表达的特点及临床意义。【方法】选取我科收治的64例原发性膀胱癌患者作为实验组(浅表性46例,浸润性18例),对照组为正常人群,共15例。分别取外周血,通过流式细胞仪检测CD8^+T淋巴细胞表明PD-1的表达量;另外取肿瘤患者膀胱癌组织和正常人群的膀胱肿瘤行免疫组化检测膀胱细胞或膀胱肿瘤细胞表面PD-L1的表达情况。【结果】膀胱癌患者外周血中CD8^+T淋巴细胞PD-1的表达量明显高于对照组[(2.25±0.60)%vs(0.68±0.17)%,P<0.001],其中浸润性膀胱癌患者外周血T细胞PD-1的表达高于浅表性膀胱癌患者[(3.04±0.46)%vs(0.68±0.17)%,P<0.001];膀胱肿瘤细胞和正常膀胱组织细胞PD-L1的表达率分别为:40.6%(26/64)和0(0/15,P<0.001),其中浸润性膀胱肿瘤细胞与浅表性膀胱肿瘤细胞PD-L1的表达率无明显差异(41.3%vs38.8%,P>0.01)。【结论】外周血中PD-1在CD8^+T细胞的表达水平可间接反映膀胱癌的进展程度,膀胱肿瘤细胞表面高表达PD-L1,但与肿瘤的进展无相关。[ Objective ] To investigate the characteristics of PD-1/PD-L1 expressed on T cell and bladder cancer cell and clini- cal significance. [ Methods ] 64 patients with primary bladder cancer were into experiment group and 10 normal people were into con- trol group. Peripheral bloods were used to test the PD-1 expressed on CD8^＋T lymphocytes by flow cytometry. Immunohistochemistry staining was used to detect the PD-L1 expression in tumor and normal specimen. [ Results ] PD-1 expressed on CD8^＋T lymphocytes was （2.25 ± 0.60）% in experiment group and （0.68 ± 0.17）% in control group, respectively （P 〈 0.001 ）. And the PD-1 expression on T ceil in invasive bladder cancer patient was significant higher than superficial bladder cancer patients [ （3.04 ± 0.46）% vs （0.68 ± 0.17）%, P 〈 0.0011. The expression of PD-L1 in experiment group was higher than control group, （26/64 vs 0/15, P 〈 0.001 ）. But there was no different between invasive and superficial bladder cancer patients, （41.3% vs 38.8%, P 〉 0.01 ）. [ Conclusions ] Expression of negative stimulatory molecule PD- 1 in CD8^＋T lymphocytes of peripheral blood is significantly correlated with bladder cancer advanced. Bladder cancer cell was strongly expressed PD-L1, and this expression is not related to cancer advanced.

关 键 词：PD-1/PD-L1 CD8^+T细胞 膀胱肿瘤细胞 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]