芍药内酯苷对放射线辐照法致血虚免疫抑制小鼠的补血作用及机制研究  

Effects and mechanism of paeoniflorin on mouse model of blood deficiency syndrome induced by radioactive rays

作　　者：王成龙[1] 王林元[1] 朱映黎[1] 瞿研[1] 吴丽[1] 王莎[1] 费文婷 张建军[1] 

机构地区：[1]北京中医药大学中医学院,硕士研究生100029

出　　处：《环球中医药》2017年第7期782-787,共6页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81473370)

摘　　要：目的研究芍药内酯苷对放射线辐照诱导血虚小鼠的补血作用及调控机制。方法采用放射线辐照法复制小鼠血虚模型,以四物颗粒和芍药苷为阳性对照药,灌胃芍药内酯苷,检测外周血象、脾脏指数及分离血清,用放射免疫法检测其白细胞介素-1、粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)、肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)的含量变化并采用实时荧光定量聚合酶链式反应法检测脾脏中GM-CSF和TNF-α的基因表达。结果与模型组比较,芍药内酯苷30 mg/kg组对白细胞数、脾脏指数均有升高作用(P<0.05),对GM-CSF含量水平亦有升高作用(P<0.05),但降低TNF-α的含量水平(P<0.01)及脾脏中TNF-αmRNA的表达(P<0.01)。结论芍药内酯苷作为白芍的特征性有效成分,通过对造血细胞因子的调控作用对抗放射线辐照所致的血虚状态,提示其为白芍养血敛阴功效的物质基础之一。Objective To explore the effects and mechanism of paeoniflorin on mouse model of blood deficiency syndrome induced by 60Co-γ irradiation.Methods Mouse model of blood deficiency syndrome induced by 60Co-γ irradiation, Siwu granules and albiflorin was regarded as positive control.Paeoniflorin was given during modeling.The peripheral hemogram and index of thymus gland and spleen were detected, the levels of IL-1, GM-CSF, and TNF-α in serum were detected by radioimmunoassay.The mRNA expressions of hematopoietic-related factors in spleen were detected by RT-qPCR.Results Compared with the model group, the amount of WBC and the index of spleen in paeoniflorin group at the dose of 30 mg/kg were increased obviously （P〈0.01, P〈0.05） and the levels of GM-CSF in serum in paeoniflorin group（30 mg/kg） was increased obviously （P〈0.01, P〈0.05）, but the level of TNF-α in serum in groups of 30 mg/kg paeoniflorin were reduced （P〈0.05） and the expression of TNF-α mRNA in spleen were decreased （P〈0.01）.Conclusion Paeoniflorin is the characteristic effective constituents of paeoniae radix alba, has regulating effect on hematopoietic cytokine to against blood deficiency induced by radioactive rays.It reminders that paeoniflorin is one of the basic nourishing materials basis for nourishing blood and astringing yin in paeoniae radix alba.

关 键 词：芍药内酯苷 放射线辐照法 血虚证 造血细胞因子 

分 类 号：R364.7[医药卫生—病理学]