当归、薄荷挥发油提取及包合工艺研究  

Study on the extraction and inclusion process of volatile oil from angelicae sinensis radix and mentha canadensis

作　　者：张玲玲[1] 倪健[1] 杜雪莹[1] 张欣[1] 张娟[1] 王海涛[1] 杨春静[1] 付京[1] 张淼[1] 郝晓凤 谢立科 曲昌海[1] 

机构地区：[1]北京中医药大学中药学院,硕士研究生100102 [2]中国医学科学院眼科医院内障眼病2科

出　　处：《环球中医药》2017年第7期808-812,共5页Global Traditional Chinese Medicine

基　　金：北京市科学技术委员会"十病十药"专项基金(Z141100002214001)

摘　　要：目的优选当归、薄荷挥发油的提取及β-环糊精包合工艺。方法采用水蒸气蒸馏法进行提取,以加水倍量、蒸馏时间为考察因素,以挥发油体积为评价指标。包合工艺以温度、搅拌时间、挥发油与β-环糊精和水的配比(m L∶g∶m L)为考察因素,以挥发油包合率和包合物产量为评价指标,采用饱和水溶液法,以正交试验优选包合工艺。采用X射线衍射法和显微镜法对包合物进行验证。结果优选的提取工艺为当归、薄荷加8倍量水,提取6小时;包合工艺为温度30℃,搅拌3小时,挥发油∶β-CD∶水=1∶6∶60(m L∶g∶m L)。验证结果显示包合物形成。结论所选工艺合理、可行,可用于当归、薄荷中挥发油的提取及挥发油与β-环糊精的包合。Objective To optimize the extraction process of essential oil from Angelica sinensis and Mentha Canadensis and the inclusion process of beta cyclodextrin.Methods Steam distillation was used to extract, the amount of water and distillation time were taken as the research factors, and the volume of volatile oil was used as the evaluation index.Temperature, mixing time, the ratio of volatile oil, beta cyclodextrin and water （mL∶g∶mL） were taken as the research factors of inclusion process.The inclusion rate and inclusion yield of volatile oil were used as evaluation indexes, and saturated water solution method was used, the inclusion process was optimized by orthogonal test.The inclusion complexes were verified by X ray diffraction and microscopy.Results The optimized extraction conditions were 8 times amount of water, extracting for 6 hours.The optimized inclusion conditions were the temperature is 30℃, stirring for 3 hours, the volatile oil∶beta-CD∶ water =1∶6∶60 （mL∶g∶mL）.Validation results showed inclusion complexes formed.Conclusion The process is reasonable and feasible, and can be used for the extraction of volatile oil from Angelica sinensis and Mentha Canadensis,, and be used for the inclusion of volatile oil and beta cyclodextrin.

关 键 词：当归 薄荷 挥发油 提取工艺 包合工艺 Β-环糊精 

分 类 号：R284.2[医药卫生—中药学]