MicroRNA-29b调控胶质瘤发生的机制研究  

Study on the expression and function of microRNA-29b in human glioma

作　　者：侯文仲[1] 许远鹏 毛振敏[1] 陈子阳[1] 曾敏敏[1] 李在雨[1] 

机构地区：[1]广州医科大学第六附属医院(清远市人民医院)神经外科,广东清远511518

出　　处：《转化医学电子杂志》2017年第7期44-47,共4页E-Journal of Translational Medicine

基　　金：清远市科技计划项目(2016B025)

摘　　要：目的:探讨microRNA-29b调控胶质瘤发生的机制.方法:利用realtime-PCR检测不同来源的胶质瘤组织和胶质瘤细胞中miR-29b的表达,MTT检测miR-29b对胶质瘤细胞增殖的影响;流式细胞术检测miR-29b对胶质瘤细胞增殖周期的影响;Western blot检测miR-29b对细胞周期调控蛋白cyclin D1和cyclin E表达的影响.结果:MiR-29b在胶质瘤中低表达,过表达miR-29b能够抑制U87和U251胶质瘤细胞的增殖,miR-29b使得胶质瘤G1期细胞增多,S期细胞减少,并且抑制cyclin D1和cyclin E的表达.结论:MiR-29b能够抑制胶质瘤细胞的增殖,调控胶质瘤细胞的细胞周期,是新的胶质瘤生长抑制因子.AIM： To study the function and regulation mecha- nism of miRNA-29b in hunman glioma. METHODS： Realtime PCR was used to detect miR-29b expression in glioma tissues, and MTI＂ was used to detect the influence of miR-29b on glioma cell proliferation. Flow cytometry was used to detect the influence of miR-29b on cell cycle; Western blot was used to detect the influence of miR-29b on the expression of eyclin D1 and cyclin E. RESULTS： MiR-29b was low expression in glioma tissues, and overexpression of miR-29b could inhibit the proliferation of U87 and U251 glioma ceils, miR-29b can increase the number of G1 phase cells and reduce the number of S phase ceils, and inhibit the expression of eyclin D1 and cyclin E. CONCLUSION： MiR- 29b can regulate glioma cell cycle, inhibit the proliferation of gli- oma cells, and miR-29b may be a tumor suppressor gene in human glioma.

关 键 词：microRNA-29b 胶质瘤 增殖 细胞周期 治疗靶点 

分 类 号：R739.41[医药卫生—肿瘤]