长链非编码RNA HULC在胃癌中的表达及其分子机制  

Clinical significance of expression of long non-coding RNA HULC in gastric carcinoma

作　　者：倪曙民[1] 叶孟[1] 

机构地区：[1]宁波大学医学院附属医院肿瘤内科,浙江省宁波市315020

出　　处：《世界华人消化杂志》2017年第18期1667-1670,共4页World Chinese Journal of Digestology

基　　金：浙江省自然科学基金资助项目;No.LY16H160005~~

摘　　要：目的探讨长链非编码RNA HULC在胃癌中的表达及分子机制.方法取2012-12/2017-02医院收治胃癌患者42例,患者均行手术治疗,取患者癌组织与癌旁组织,采用实时荧光定量PCR技术测定癌症组织与癌旁组织中长链非编码RNA HULC表达水平,进一步分析与临床病理资料的关系.结果 42例胃癌组织中长链非编码RNA HULC表达水平高于癌旁组织(0.41±0.12 vs 0.20±0.10,P<0.05);胃癌组织中长链非编码R N A HULC表达与性别、年龄、肿瘤大小及肿瘤位置差异无统计学意义(P>0.05);长链非编码RNA HULC表达与胃癌的分化程度、淋巴结转移及TNM分期关系密切(P<0.05).多因素Logistic回归分析显示:长链非编码RNA HULC表达与胃癌的分化程度、淋巴结转移及TNM分期关系密切(P<0.05).结论在胃癌组织中长链非编码RNA HULC表达呈高表达,并且与临床病理资料具有紧密的联系,能判断胃癌肿瘤的恶性程度,指导临床治疗.AIM To detect the expression of long non-coding RNA HULC in gastric carcinoma and to analyze its clinical significance.METHODS From December 2012 to February 2017, 42 patients with gastric cancer treated surgically were included in this study. Cancer tissues and tumor adjacent tissues were collected from these patients. Real-time quantitative PCR was used to determine the expression of HULC, and the relationship of HULC expression with clinical and pathological data was analyzed.RESULTS The expression of HULC in gastric carcinoma tissues was significantly higher than that in tumor adjacent tissues（0.41 ± 0.12 vs 0.20 ± 0.10, P〈0.05）. HULC expression was significantly correlated with degree of tumor differentiation, lymph node metastasis and TNM stage（P〈0.05）, but not with gender, age, tumor size or location（P〈0.05）. Multivariate logistic regression analysis showed that HULC expression was closely related to degree of tumor differentiation, lymph node metastasis and TNM stage（P〈0.05）.CONCLUSION The expression of HULC in gastric carcinoma is high, and it is closely related to degree of tumor differentiation, lymph node metastasis and TNM stage. HULC expression can be used to judge the malignancy of gastric cancer and guide clinical treatment.

关 键 词：长链非编码RNA HULC 癌组织 癌旁组织 实时荧光定量PCR技术 分子机制 

分 类 号：R735.2[医药卫生—肿瘤]