角膜移植术后内皮型免疫排斥反应的活体共聚焦显微镜分析  

In vivo confocal microscopy analysis of different endothelial immune rejection after corneal transplantation

作　　者：侯飞[1] 黎明[1] 方敏[1] 

机构地区：[1]暨南大学附属深圳市眼科医院深圳眼科学重点实验室,深圳518040

出　　处：《齐齐哈尔医学院学报》2017年第10期1143-1144,共2页Journal of Qiqihar Medical University

基　　金：深圳市卫生计生系统科研项目(201401064)

摘　　要：目的研究分析角膜移植术后内皮型免疫排斥反应的活体共聚焦显微镜。方法选择2013年10月至2015年11月本院收治的32例行角膜移植术患者为研究对象,应用激光共聚焦显微镜于治疗前后对病灶特点位点实施检查,观察分析检查情况。结果共聚焦显微镜显示,治疗前16例患者可发现典型内皮排斥线,另外16例患者呈植片弥漫水肿混浊。治疗后,23例患者好转,激光共聚焦显微镜可清晰的发现基质细胞核,且内皮细胞表面免疫细胞个数减少,上皮基底层下炎性细胞也明显减少。结论激光共聚焦显微镜可动态观察角膜移植术后内皮型免疫排斥反应过程及转归,对疾病治疗具有一定的参考意义。Objective To investigate the in vivo confocal microscopy of different endothelial immune rejection after corneal transplantation. Methods From October 2013 to November 2015,32 patients with corneal transplantation were selected as the subjects in our hospital,the characteristics and sites of the lesions were examined before and after the treatment with confocal laser scanning microscope,the examination was analyzed.Results After analysis,32 patients with 16 cases can be found in typical endothelial rejection line,another 16 cases were graft diffuse edema and opacity. After treatment,23 cases of patients improved,under the laser scanning confocal microscopy can be clearly found in the nuclear matrix,and endothelial cells on the surface of immune cells to reduce the number of,Picchi bottom dendritic Langerhans cells also significantly reduced.Conclusions Using laser scanning confocal microscopy to observe the endothelial immune rejection process and the outcome after corneal transplantation can provide reference materials for the treatment of the disease,which is of great significance.

关 键 词：角膜移植 活体 共聚焦显微镜 内皮型免疫排斥反应 

分 类 号：R779.65[医药卫生—眼科]