盐霉素纳米结构脂质载体冻干粉的制备  

Preparation of salinomycin loaded nanostructured lipid carrier lyophilized powder

作　　者：金珊珊[1,2] 马晓星[2] 袁橙[2] 董岩[2] 韩翠艳[2] 

机构地区：[1]佳木斯大学药学院,黑龙江154000 [2]齐齐哈尔医学院药学院

出　　处：《齐齐哈尔医学院学报》2017年第10期1193-1196,共4页Journal of Qiqihar Medical University

基　　金：黑龙江省自然科学基金(H2015070)

摘　　要：目的制备盐霉素纳米结构脂质载体(Sal-NLCs)冻干粉。方法单因素考察冻干保护剂种类、剂量、加入方式、预冻方法等因素对Sal-NLCs粒径、多分散指数(PDI)和Zeta电位的影响,确定最佳冻干工艺,并测定Sal-NLCs冻干粉的制剂学性质进行考察。结果最佳冻干工艺:将5%的蔗糖冻干保护剂加入到制备好的Sal-NLCs溶液中,分别经-4℃、-20℃、-80℃预冻4 h、10 h、10 h,并冷冻干燥24 h。制备得到的冻干粉复溶后,粒径、PDI、Zeta电位及包封率较冻干前无明显变化。且冻干粉室温放置4周,体积不变,外观饱满紧凑。结论按照最佳冻干工艺制备的Sal-NLCs冻干粉性状稳定。Objective To prepare salinomycin loaded nanostructured lipid carriers lyophilized powder.Methods The cryoprotector types,dosage,adding method was investigated by single factor method with particle size,Zeta potential and encapsulation efficiency of Sal-NLCs as evaluating indicator to determine the best freezedrying technology. The properties of Sal-NLCs freeze-dried powder was also studied. Results 5% of sucrose was added into the prepared Sal-NLCs solution. After a series of pre-freezing process,such as-4 ℃ for 4h,-20 ℃for 10 h,-80 ℃ for 10 h,the Sal-NLCs solution was transferred to freeze dryer immediately. The freeze dried powder prepared was well-stacked and there was no obvious change in particle size,Zeta potential and entrapment efficiency after rehydration. The powder still remained stable after it was placed at room temperature for 4 weeks.Conclusions The salinomycin loaded nanostructured lipid carriers lyophilized powder prepared by the optimum freeze-drying process are stable.

关 键 词：盐霉素 纳米结构 脂质载体 冻干保护剂 

分 类 号：R94[医药卫生—药剂学]