意大利蜜蜂6日龄幼虫肠道内球囊菌的差异表达基因分析  

Transcriptome analysis of differentially expressed genes in Ascosphaera apis stressing the 6-day-old larval gut of Apis mellifera ligustica

作　　者：杜宇[1] 熊翠玲[1] 史秀丽 郑燕珍[1] 付中民[1] 徐细建[1] 陈大福[1] 郭睿[1] 

机构地区：[1]福建农林大学蜂学学院,福建福州350002 [2]新疆维吾尔自治区蜂业发展中心,新疆乌鲁木齐830000

出　　处：《浙江农业学报》2017年第7期1119-1128,共10页Acta Agriculturae Zhejiangensis

基　　金：现代农业产业技术体系建设专项资金(CARS-45-KXJ7);福建农林大学科技发展资金(KF2015123);国家级大学生创新创业项目(201610389016)

摘　　要：为检测蜜蜂球囊菌(Ascosphaera apis)在胁迫意大利蜜蜂(Apis mellifera ligustica,简称意蜂)幼虫后期的基因表达谱,利用RNA-seq技术对纯培养的球囊菌孢子及球囊菌胁迫的意蜂6日龄幼虫肠道进行深度测序。通过比较处理组和对照组得到21 361个差异表达基因(DEGs),包括15 306个上调基因和6 055个下调基因。GO富集分析结果显示,上调基因富集于39个GO条目(term),基因富集数最多的为细胞进程(2 416unigenes)、细胞(2 408 unigenes)和细胞组件(2 408 unigenes);下调基因富集于38个GO term,基因富集数最多的为代谢进程(1 227 unigenes)、细胞进程(1 185 unigenes)和细胞(1 131 unigenes)。KEGG代谢通路富集分析结果显示,上调基因富集在119个通路,其中基因富集数最多的是核糖体(221 unigenes),其次为内质网中蛋白加工(190 unigenes)和内吞作用(177 unigenes);下调基因富集在113个通路上,基因富集数最多的是核糖体(144 unigenes),其次为RNA转运(113 unigenes)和氨基酸生物合成(108 unigenes)。进一步分析发现,富集在MAPK信号通路上的64个基因上调表达,说明该通路在球囊菌胁迫后期被激活。研究结果不仅为揭示球囊菌在胁迫意蜂幼虫后期的病原-宿主互作提供了重要信息,也为阐明球囊菌致病的分子机制奠定了基础。The purified Ascosphaera apis spores and the 6 -day-old larval gut of Apis mellifera ligustica under the stress of A. apis were sequenced using RNA-seq technology to detect gene expression patterns of the fungal pathogen. A to-tal of 21 361 differentially expressed genes （DEGs） were obtained, including 15 306 up-regulated genes and 6 055 down-regulated genes. GO enrichment analysis showed that the up-regulated genes were enriched in 39 terms, among them the mostly enriched ones were cellular process （2 416 unigenes） , cell （2 408 unigenes） and cell part （2 408unigenes） ; the down-regulated genes were enriched in 38 terms, and the mostly enriched ones were metabolic process （1 227 unigenes） , cellular process （1 185 unigenes） and cell （1 131 unigenes）. KEGG enrichment analy-sis displayed that the up-regulated genes were enriched in 119 pathways, and the largest groups were ribosome （221 unigenes） , protein processing in endoplasmic reticulum （ 190 unigenes） and endocytosis （177 unigenes） ; the down- regulated genes were enriched in 113 pathways, and the mostly enriched ones were ribosome （ 144 unigenes） , RNA transport （113 unigenes） as well as biosynthesis of amino acid （108 unigenes）. Furthermore, 64 up-regulated genes were found to be enriched in MAPK signaling pathway, suggesting that it was induced to a large extent. The data not only provided key information for uncovering the pathogen-host interaction during the late stage of the stress of A. apis on A. m. ligustica larvae, but also laid some foundation for clarifying molecular mechanisms regulating the pathogen-esis of A. apis.

关 键 词：球囊菌 意大利蜜蜂 幼虫肠道 差异表达基因 RNA-SEQ 

分 类 号：S895.1[农业科学—特种经济动物饲养]