TNF-α和IL-10基因多态性及其血浆浓度与弥漫大B细胞淋巴瘤的关系  

Correlation between polymorphisms in TNF-α and IL-10 genes and plasma cincentration with diffuse large B-cell lymphoma

作　　者：沈艳[1] 顾霞[2] 富玲[3] 邹红云[4] 石雨薇[5] 

机构地区：[1]广州医科大学附属第四医院病理科,广州511447 [2]广州医科大学附属第一医院病理科,广州510120 [3]新疆维吾尔自治区人民医院血液科,乌鲁木齐830001 [4]兰州军区乌鲁木齐总医院临床医学研究所,乌鲁木齐830000 [5]新疆医科大学第二附属医院血液科,乌鲁木齐830063

出　　处：《临床与实验病理学杂志》2017年第7期715-719,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(30860096)

摘　　要：目的探讨TNF-α和IL-10基因单核苷酸多态性(single nucleotide polymorphisms,SNPs)、血浆浓度与弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)三者之间的关系。方法采用SNa Pshot SNP分型技术检测38例DLBCL患者及77例健康对照者TNF-α和IL-10基因6个位点SNPs,并应用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)测定血浆TNF-α和IL-10浓度。结果 DLBCL组血浆IL-10水平明显高于对照组(P<0.05),TNF-α水平在两组间差异无明显相关性(P>0.05)。在8个候选的SNPs位点中,TNF-α-863C/A多态性分布在DLBCL组和对照组间差异有显著性,其中CA/AA基因型携带者罹患DLBCL的风险为CC基因型的2.280倍(95%CI=1.091~4.765,P=0.028),其余7个SNPs多态性分布在两组间差异均无显著性(P>0.05)。在排除患病状况对血浆TNF-α和IL-10水平的影响后,各基因多态性对其相应血浆浓度均无明显影响(P>0.05)。结论 TNF-α-863C/A位点多态性与DLBCL易感性有关,IL-10可能在DLBCL发生、发展中起重要作用。Purpose To explore the association between single nucleotide polymorphisms （SNPs） of TNF-α and IL-10 genes as well as their plasma levels and pathogenesis of diffuse large B-cell lymphoma （DLBCL）. Methods Snapshot SNP genotype technique was used to assess genetic variation in 6 SNPs for TNF-α and IL-10 in 38 DLBCL cases and 77 healthy controls, and enzyme-linked immunosorbent assay （ELISA） was used to measure the plasma coneentrations of TNF-α and IL-10 in the above population. Results Plasma IL-10 level in DLBCL cases was higher than that in controls （ P 〈 0. 05 ） , but there was no significant difference in plasma TNF-αlevel between the two groups （P 〉0.05）, Of all the candidate SNPs, the geneotype distribution for TNF-t^-863C/A showed significant differences between the case and control groups （P 〈 0. 05 ）, and carriers with CA/AA genotypes had more two-fold risks of DLBCL than those with the CC genotype （95% CI=1. 091-4. 765, P = 0. 028）. However several other candidate SNPs showed no sig- nificant difference in the geneotype distributions between the ca- ses and controls （P 〉 0. 05）. After excluding the effects of dis- ease status on plasma TNF-alpha and IL-10 levels, the polymor- phism of each gene had no significant effect on the corresponding plasma concentration （ P 〉 0. 05 ）. Conclusion TNF-α-863C/ A polymorphism may be associated with the risk of DLBCL, and IL-10 play an important rule in occurrence and development of DLBCL.

关 键 词：弥漫大B细胞淋巴瘤 TNF-Α IL-10 单核苷酸多态性 酶联免疫吸附法 

分 类 号：R733.4[医药卫生—肿瘤]