HOTAIR-siRNA通过miR-21对肝癌侵袭、转移的影响  

Effect of HOTAIR-siRNA on invasion and metastasis of hepatocellular carcinoma by miR-21

作　　者：王永玲[1] 高建芝[2,3] 闫姝洁 王琼[3] 韦立新[3] 

机构地区：[1]新乡医学院基础医学院,新乡453003 [2]中国人民解放军总医院合作医院涿州市医院肿瘤科,涿州072750 [3]中国人民解放军总医院病理科,北京100080

出　　处：《临床与实验病理学杂志》2017年第7期720-723,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：全国博士后56批二等资助(20140435);河北省保定知识产权局资助(15ZF062)

摘　　要：目的探讨长链非编码HOTAIR靶控miR-21对肝癌细胞侵袭的影响及相关机制。方法采用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)技术检测30例肝癌组织中miR-21的表达,及其与临床病理特征的相关性;HOTAIR的siRNA转染人肝癌HepG2细胞,采用Transwell小室法检测HepG2细胞的侵袭活性;应用qRT-PCR法检测HepG2细胞中miR-21的表达;采用Western blot法检测MMP-2蛋白的表达,并分析其与miR-21的相关性。结果 qRT-PCR检测发现miR-21在肝癌组织中的表达水平均明显高于癌旁组织(P<0.05);miR-21表达与肿瘤恶性程度、分化程度及侵袭转移均呈正相关;与患者AFP、肿瘤大小及肿瘤数目无关。沉默HOTAIR基因后,HepG2细胞侵袭能力下降,miR-21基因和MMP-2蛋白的表达均下调且两者呈正相关。结论 miR-21在肝癌组织中呈高表达,并与肝癌恶性程度、肿瘤分化、门静脉癌栓及侵袭转移有关;HOTAIR可能通过靶控miR-21的表达抑制肝癌细胞的侵袭与转移。Purpose To study the role of lnc-HOTAIR and its target-controlled miR-21 on invasion and metastasis of hepa-tocellular carcinoma cells （ HCC ） and its related mechanisms. Methods The expression of miR-21 in 30 cases of HCC was detected by quantitative real-time PCR （qRT-PCR）, and corre-lation with clinical pathology was also analyzed, siRNA of lnc-HOTAIR was transfected to HCC HepG2 cell （ HepG2）, invasive activities of HepG2 was detected by Transwell, the expres- sion of miR-21 was determined by qRT-PCR, the expression of protein MMP-2 was determined by Western blotting, and the correlation with miR-21 expression was analyzed. Results Compared with pericarcinous tissue, the expressions of miR-21 in tumor tissue increased apparently （ P 〈 0. 05 ）. The expressions of miR-21 correlated positively with malignancy degree,differentiation degree and invasion and metastasis respectively, and correlated negatively with tumour size, tumour number and level of AFP respectively. After silencing the HOTAIR gene, the invasive activities of HepG2 were suppressed, the expression of miR-21 gene and MMP-2 protein were down regulated, and both of them were positively correlated. Conclusion High expressions of miR-21 in liver cancer correlate positively with malig- nancy degree, differentiation degree, intrahepatie and extrahepatic metastases and portal vein tumor thrombus respectively. HOTAIR may inhibit the invasion and metastasis of HCC by controlling the expression level of miR-21.

关 键 词：肝肿瘤 长链非编码RNA MIR-21 侵袭和转移 MMP-2 

分 类 号：R735.7[医药卫生—肿瘤]