精神分裂症患者中miR-197-3p调控瘦蛋白水平的研究  

The miR-197-3p Regulating Leptin Level in Schizophrenia Patients

作　　者：郭天虹 罗彬瑞 王雨涵 彭瑛 白克镇 黄远帅 Guo Tianhong Luo Binrui Wang Yuhan Peng Ying Bai Kezhen Huang Yuanshuai(Department of Transfusion, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China Department of Etiologies, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China Department of Psychiatry, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China)

机构地区：[1]西南医科大学附属医院输血科,泸州646000 [2]西南医科大学附属医院检验科,泸州646000 [3]西南医科大学附属医院精神科,泸州646000

出　　处：《中国细胞生物学学报》2017年第6期772-778,共7页Chinese Journal of Cell Biology

基　　金：四川省卫生厅2012年科研课题(批准号:120336);泸州市科技局计划项目[批准号:2012-S-37(11/29)]资助的课题~~

摘　　要：该文主要分析了精神分裂症(schizophrenia,SCZ)患者血清中候选微RNA(microRNA,miRNA)水平的变化,研究其作用靶点及机制。针对前期芯片结果分析发现的差异表达miRNA,该研究使用荧光定量PCR对一批患者和对照血清进行验证分析。利用生物信息学工具分析经验证的差异表达miRNA,寻找潜在的作用靶点。进一步对miRNA及其潜在靶点进行调控功能的验证。芯片筛选结果发现,microRNA-197-3p(miR-197-3p)在初诊患者中呈低表达,较临床痊愈者和健康人有显著性差异,并在45例初诊患者和50例健康人对照血清中得到验证。生物信息学分析发现,瘦蛋白(leptin)mRNA可能是miR-197-3p的一个作用靶点,并经ELISA验证患者相对于健康人血清中瘦蛋白浓度显著升高。细胞水平的研究发现,miR-197-3p模拟物可以下调瘦蛋白水平,相反miR-197-3p抑制剂可以上调其水平。荧光素酶活性实验证实,miR-197-3p可能通过结合于瘦蛋白mRNA 3′UTR(3′untranslated region)的特异性位点,调控其水平。该研究表明,miR-197-3p水平下降,进一步上调瘦蛋白的水平可能在精神分裂症发病过程中发挥一定作用。We analyzed the candidate microRNA（miRNA） levels in the serum of schizophrenia（SCZ）patients and controls, and explored the target mRNA of candidate miRNAs. Candidate mi RNAs found by miRNA array were analyzed by Real-time PCR in a cohort including patients and healthy controls. The potential target mRNAs of mi RNAs were analyzed by bio-informatics tools and studied by in vitro experiments. microRNA-197-3p（miR-197-3p） was found down-regulated in patients compared to controls by Real-time PCR. Leptin was found to be one of the targets of miR-197-3p by online bio-informatics tools. Leptin concentration was found up-regulated significantly in patients＇ serum by ELISA. In vitro study illustrated that mi R-197-3p targeted 3′ untranslated region（3′UTR） of leptin mRNA and reduced its expression. The pathway mi R-197-3p regulating leptin may play an important role in schizophrenia pathogenesis.

关 键 词：miR-197-3p 瘦蛋白 精神分裂症 

分 类 号：R749.3[医药卫生—神经病学与精神病学]