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作 者:周田园 李德超[1] 李慕勤[1] 李善昌[1] 刘爽[1] 马菁[1] 田垒[1] 于佳莹[1] 温鑫[1] ZHOU Tian -yuan L I De-chao Li Mu-qin Li Shan-chang Liu Shuang Ma Jing Tian Lei Yu Jia-ying Wen Xin(Key Biomedical Materials Laboratory of Colleges and Universities in Heilongjiang Province, Jiamusi University, Jiamusi 154007, China)
机构地区:[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江佳木斯154007
出 处:《口腔医学研究》2017年第7期703-706,共4页Journal of Oral Science Research
基 金:国家自然科学基金项目(编号:31370979);佳木斯大学大学生科技创新项目(编号:xsym 2016-07);佳木斯大学口腔医学预防与治疗协同创新中心(编号:2011xtcx 2016-02)
摘 要:目的:探讨纯钛种植体表面加载环型多肽对成骨细胞生物行为的影响。方法:微弧氧化组为M组,微弧氧化加载多巴胺组为P组,微弧氧化加载多巴胺接枝环环肽组为R组。扫描电镜对3组钛片进行表面形貌分析,能谱仪分析3组试件元素成分。CCK-8检测成骨细胞不同时间点在3组的增殖黏附能力,激光共聚焦观察成骨细胞在3组涂层表面的铺展情况。结果:扫描电镜及能谱显示涂层加载成功,CCK-8显示R组的A值在各个时间点上都高于M组和P组,且各组比较具有统计学意义(P<0.05)。激光共聚焦观察R组细胞数目最多,伪足较多,细胞联系最为紧密。结论:纯钛表面加载环肽后能有效的促进成骨细胞早期的黏附及增殖。Objective:To discuss the effect of Cyclo(RGD)coating on the surface of pure titanium implant on the biological behavior of osteoblasts.Methods:The specimens were divided into 3groups:pure titanium ultrasonic micro-arc-oxidation(group M);pure titanium ultrasonic micro-arc-oxidation-dopamine(group P);and pure titanium ultrasonic micro-arc-oxidation-dopamine-Cyclo(RGD)(group R).Scanning electron microscope was used to observe the surface morphology.The surface elements of three groups were analyzed by energy spectrometer.CCK-8experiment was used to detect the adhesion rate and proliferation of osteoblasts in different time points.The spread of osteoblasts on the surface of three groups was observed by laser confocal microscopy.Results:The observation of scanning electron microscope and the spectrum of coating displayed successful loading.And the results of CCK-8showed that the OD value of group R was higher than those of group M and group P on tested time points(P〈0.05).Confocal laser microscope showed the cytoskeleton form.During the same period,the spread area and the number of microfilament and pseudopodia of both group P and group R were better than those of group M.And the group R was the most conducive to cell stretch.Conclusion:The ultrasonic micro-arc oxidation-dopamine-cyclo(RGD)coating showed a good effect on early adhesion and proliferation of osteoblasts.
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