下调PI3K/Akt信号通路对抑制喉癌细胞生长的影响  

Effects of down-regulation of PI3K/Akt signaling pathway on growth of laryngeal cancer cells

作　　者：王慧敏[1] 余文发[1] 周航[1] 卢振民[1] 

机构地区：[1]新乡医学院第一附属医院耳鼻咽喉科,河南卫辉453100

出　　处：《重庆医学》2017年第21期2892-2894,共3页Chongqing medicine

基　　金：河南省重点科技攻关项目(152102310355)

摘　　要：目的探讨钾通道阻断剂四乙胺(TEA)通过磷脂酰肌醇3激酶(PI3K)/丝氨酸苏氨酸蛋白激酶(Akt)信号通路对人喉癌上皮细胞(Hep-2)增殖与凋亡的影响。方法将TEA作用于Hep-2,用噻唑蓝(MTT)法测定Hep-2细胞活性,计算出TEA对Hep-2的增殖抑制率;采用Hoechest33258染色检测细胞凋亡,用流式细胞仪(FCM)法测定Hep-2凋亡率。结果 5、10、20mmol/L TEA接种后,Hep-2细胞增殖抑制率分别达到12.573%、31.385%和56.132%,Hep-2作用96h后细胞凋亡率分别为(41.64±2.67)%、(58.76±4.32)%和(72.65±6.54)%,TEA处理组抑制率和凋亡率都高于未用TEA处理的对照组,差异均有统计学意义(P<0.05)。结论 TEA可抑制Hep-2的增殖及体内生长,促进Hep-2的凋亡。Objective To investigate the effect of potassium channel blocker tetraethtylamine （TEA） on the proliferation and apoptosis of human laryngeal carcinoma cells （Hep-2） through the PI3K/Akt signaling pathway. Methods TEA acted on HEp-2. The methyl thiazolyl blue （MTT） method was used to determine the Hep-2 cell activity and the proliferation inhibition rate of TEA on Hep-2 was calculated ; the Hoechest33258 staining was used to detect cell apoptosis,and the Hep-2 apoptosis rate was detected by flow cytometry （FCM） assay. Results After inoculation by 5,10,20 mmol/L TEA,the Hep-2 cell proliferation inhibition rate reached 12. 573% ,31. 385% and 56. 132%, respectively. After 96 h Hep-2 cell action, the cellular apoptosis rates were （41.64± 2.67）% ,（58.76±4.32）% and （72. 65±6.54）% respectively,the inhibition rate and apoptosis rate in the TEA treating group were significantly higher than those in the non-TEA treating group, the differences were statistically significant （P〈0.05）, Conclu- sion TEA can inhibit the proliferation and in vivo growth of human laryngeal carcinoma cells and promotes the apoptosis of laryn- geal cancer cells.

关 键 词：钾通道阻滞剂 喉肿瘤 上皮细胞 PI3K-AKT 细胞凋亡 

分 类 号：R739.65[医药卫生—肿瘤] R730.53[医药卫生—临床医学]