Ca^(2+),Mg^(2+),K^+及胰蛋白酶和胰蛋白酶抑制剂对中国对虾卵子激活的影响  

EFFECTS OF Ca^(2+) ,Mg^(2+) ,K^+ ,TRYPSIN AND SOYBEAN TRYPSIN INHIBITOR ON OOCYTE ACTIVATION IN PENAEUS CHINENSIS

作　　者：吴长功[1] 张晓军[1] 相建海[1] 周令华[1] 刘瑞玉[1] 

机构地区：[1]中国科学院海洋研究所

出　　处：《海洋科学集刊》1999年第1期102-107,234-238,共8页Studia Marina Sinica

基　　金：国家自然科学基金项目;39670579号;国家攀登B项目(PDB6-2)资助项目;中国科学院海洋研究所实验海洋生物学开放研究实验室资助项目

摘　　要：对虾成熟卵子的激活都是由海水引起的,海水的刺激引起成熟的卵母细胞发生皮层反应,完成减数分裂,这一过程同有无精子的作用无关(Clark et al.,1977,1980;Lynn etal.,1991;Pillai et al.,1987)。在对锐脊单肢虾(Sicyonia ingentis)的卵子激活研究中发现,其成熟卵子无论受精与否在接触海水以后均具有相同的激活过程。Effects of Mg2+,Ca2+ and K+ in artificial seawater and trypsin and soybean trypsin inhibitor (SBTI) on oocyte activation in Penaeus chinensis were studied in the spring in 1997. The results showed that Mg2+ was a necessary factor in oocyte activation,but Ca2+ and K+ were not. They had the same effects on fertilized and unfertilized eggs. Trypsin (0. 1%)could dilute the jelly layer (JL)and hatching envelope (HE) and effect the normal cleavage of fertilized eggs. SBTI (0. 01%) could completely inhibit oocyte activation and fertilization. Trypsin and SBTI had not effect on eggs 30min after the eggs were spawned. This showed that the completely formed JL and HE could protect against the effects of trypsin and SBTI. Laser scanning confocal microscope was used in comparative study on oocyte activation and cleavage characteristics of eggs in the test group and the control group.

关 键 词：蛋白酶抑制剂 卵子激活 中国对虾 人工海水 孵化膜 类胰蛋白酶 减数分裂 胶质层 未受精卵 顶体反应 

分 类 号：P[天文地球]