Cu^(2+)、Zn^(2+)对罗非鱼嗅觉中毒效应及其机理的初探  

STUDY ON THE TOXIC EFFECTS OF Cu^(2+) OR Zn^(2+) ON FISH'S (OREOCHROMIS SP.) OLFACTORY ORGAN AND THE TOXIC MECHANISM

作　　者：柴敏娟[1] 曾建平[1] 于淼[1] 

机构地区：[1]厦门大学海洋系

出　　处：《海洋科学集刊》2002年第1期253-258,共6页Studia Marina Sinica

基　　金：国家自然科学基金资助项目;39770102。

摘　　要：针对重金属对鱼类嗅觉毒性的研究,在组织学、行为学、电生理学及免疫细胞化学等方面已有报道。在所涉及的重金属中,有关Gu²⁺的研究相对多些,且侧重于Cu²⁺对鱼类嗅觉结构及功能的影响和解毒方法等研究（Rehnberg et al.,1986;Bjerselius et al.,1993;Winberg et al.,1992:Saucier et al.,1995;柴敏娟等,1999）,有关Zn²⁺The effects of copper and zinc ions on the electro-olfactogram (EOG) of Oreochromis sp. were studied by perfusing 4 different concentrations of CuSO4 and ZnSO4 solutions through the olfactory organs for 90 minutes, followed by rinsing with the clean water for 60 minutes. The EOG responses were evoked by stimulating the olfactory epithelium with 10-5mol/L-serine for 10 sec during the period. Combined with the histology study, we explored preliminarily the mechanism of poisoning.The results indicated: 1) that copper ions and zinc ions had inhibitory effect on EOG responses; the larger the concentration of the metal ions, the greater was the inhibitory effect; the inhibitory effect of copper ions was greater than that of zinc ions; 2) that during the period of washing by clean water, recoveries of olfactory responses in the lower concentration groups was rapid, the response amplitudes could recover completely, but that the recoveries of the higher concentration groups were slower and not complete; 3) that high concentration of copper ions and zinc ions caused damnification in the sensory area and insensory area of the olfactory epithelium, that the damnification by copper ions was greater than that by zinc ions.

关 键 词：CU ZN 中毒效应 嗅上皮 嗅板 行为学 免疫细胞化学 电生理学 感觉上皮 抑制作用 

分 类 号：X174[环境科学与工程—环境科学]